ADIPOSE TISSUE OF DROSOPHILA MELANOGASTER .I. AN EXPERIMENTAL STUDY OF LARVAL FAT BODY
ADIPOSE TISSUE OF DROSOPHILA MELANOGASTER .I. AN EXPERIMENTAL STUDY OF LARVAL FAT BODY
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DOI：
10.1002/jez.1401580203
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发表时间：
1965-01-01
影响因子：
--
通讯作者：
KING, RC
中科院分区：
文献类型：
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作者：
BUTTERWORTH, FM;BODENSTEIN, D;KING, RC
对黑腹果蝇3龄幼虫和成年雌性脂肪细胞进行了形态学、细胞化学和发育研究。新生和7日龄雌性成虫腹部的成虫型脂肪细胞含有脂质和糖原沉积。虽然细胞在这一时期增大了，但细胞核却没有。3龄时，脂肪细胞和细胞核的平均横截面积分别增加5倍和2倍。脂质和糖原的沉积贯穿这一时期。蛋白球首先出现在第三星的中间。它们的直径和数量增加，直到化蛹前，它们占细胞细胞质的1/4。在青虫中，存活的幼虫脂肪细胞及其细胞核的平均横截面积比3龄后期分别减少了2倍和3倍。存活的幼虫脂肪细胞含有不饱和脂滴、蛋白球和糖原沉积物，它们完全填满了剩余的细胞质区域。在成年后的第3天，这些细胞组织分解。2龄后期幼虫饥饿3 d后，脂肪体细胞的平均截面积减少10倍。尽管糖原的缺乏和脂质的大量减少，蛋白质球形成，并在饥饿的第3天占细胞质的1/4。移植实验表明，环腺在3龄后期产生的一种激素诱导了这些蛋白球的形成。
Morphological, cytochemical and developmental studies were undertaken of the adipose cells present in 3rd instar larvae and in adult females of Drosophila melanogaster. The adipose cells of the adult type in the abdomens of recently emerged and 7-day-old adult females contain deposits of lipid and glycogen. Although the cells increase in size during this period, their nuclei do not. During the 3rd instar the larval adipose cells and their nuclei undergo a 5- and 2-fold increase in average cross-sectional area, respectively. Deposits of lipid and glycogen are present throughout this period. Proteinaceous globules first appear in the middle of the 3rd instar. They increase in diameter and number until just before pupation they comprise 1/4 of the cytoplasm of the cells. In the young adult the surviving larval adipose cells and their nuclei have decreased in average cross-sectional area by 2 and 3 times, respectively, from the late 3rd instar values. The surviving larval adipose cells contain droplets of unsaturated lipids, protein globules, and deposits of glycogen which completely fill the remaining cytoplasmic area. By the 3rd day of adult life these cells histolyze. When late 2nd instar larvae are starved for 3 days, the average cross-sectional area of their fat body cells decreases 10 times. Despite the absence of glycogen and a huge decrease in lipid, protein globules form, and by the 3rd day of starvation comprise 1/4 of the cytoplasm. Transplantation experiments demonstrate that a hormone produced by the ring gland during the late 3rd instar induces the formation of these protein globules.