Rapid separation and identification of multiple constituents in Danhong Injection by ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry
Rapid separation and identification of multiple constituents in Danhong Injection by ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry
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超高效液相色谱-电喷雾电离四极杆飞行时间串联质谱快速分离鉴定丹红注射液中的多种成分
DOI：
10.1016/s1875-5364(16)60008-0
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发表时间：
2016-02-01
影响因子：
4.6
通讯作者：
Yang Hua
中科院分区：
文献类型：
--
作者：
Zhang Qun-Qun;Dong Xin;Yang Hua
为了表征和鉴定丹红注射液（DHI）中的多种成分，本研究建立并验证了快速超高效液相色谱-电喷雾电离四极杆飞行时间串联质谱（UHPLC-ESI-QTOF/MS）方法。共鉴定或初步鉴定了 63 种化合物，包括 33 种酚酸、2 种 C-糖基喹啉查尔酮、6 种黄酮 O-糖苷、4 种环烯醚萜苷、6 种有机酸、5 种氨基酸和 3 种核苷。总之，UHPLC-ESI-QTOF/MS 方法对于 DHI 等复杂草药基质中化合物的深入结构解析是有用且高效的。
To characterize and identify multiple constituents in Danhong injection (DHI), a fast ultra-high performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QTOF/MS) method was established and validated in the present study. A total of 63 compounds, including 33 phenolic acids, 2 C-glycosyl quinochalcones, 6 flavonoid O-glycosides, 4 iridoid glycosides, 6 organic acids, 5 amino acids, and 3 nucleosides, were identified or tentatively characterized. In conclusion, the UHPLC-ESI-QTOF/MS method is useful and efficient for in-depth structural elucidation of chemical compounds in complex matrices of herbal medicines such as DHI.