A Molecular Approach to Identification and Profiling of First-Line-Drug-Resistant Mycobacteria from Sputum of Pulmonary Tuberculosis Patients
A Molecular Approach to Identification and Profiling of First-Line-Drug-Resistant Mycobacteria from Sputum of Pulmonary Tuberculosis Patients
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DOI：
10.1128/jcm.06093-11
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发表时间：
2012-06-01
影响因子：
9.4
通讯作者：
Bhattacharya, Basudev
中科院分区：
文献类型：
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作者：
Chowdhury, Imran Hussain;Sen, Aditi;Bhattacharya, Basudev
应用常规和分子生物学技术检测和鉴定临床确诊肺结核患者痰标本中分枝杆菌的耐药性。ZN染色、培养、多重PCR和限制性片段长度多态性(RFLP)检测分枝杆菌的敏感性分别为27.7%、19.9%、92.9%和95.7%，但均具有100%的特异性。常规方法和多重等位基因特异性聚合酶链式反应(MAS-PCR)方法分别有19.3%和86.9%的病例耐药。我们证明了分子技术在结核病的准确诊断中具有潜力。
Conventional and molecular techniques were applied to detect and characterize drug resistance of mycobacteria in the sputum samples of clinically confirmed tuberculosis. The sensitivities of mycobacterium detection by ZN staining, culture, multiplex PCR, and restriction fragment length polymorphism (RFLP) were 27.7%, 19.9%, 92.9%, and 95.7%, respectively, but all were 100% specific. The conventional and multiple-allele-specific PCR (MAS-PCR) methods enabled establishment of the drug resistance in 19.3% and 86.9% cases, respectively. We demonstrated that molecular techniques have potential in the accurate diagnosis of tuberculosis.