Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle
Proteome dynamics during contractile and metabolic differentiation of bovine foetal muscle
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DOI：
10.1017/s1751731109004315
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发表时间：
2009-07-01
期刊：
影响因子：
3.6
通讯作者：
Picard, B.
中科院分区：
文献类型：
--
作者：
Chaze, T.;Meunier, B.;Picard, B.
牛肌肉的收缩和代谢特性在肉的感官品质，特别是嫩度中起着重要的作用。早期基于肌球蛋白重链异构体分析和糖酵解和氧化酶活性测量的研究表明，牛胎儿生命的第三个月以收缩和代谢分化为特征。为了完成这一数据并获得这一阶段及其调控的精确视图，我们对Charolais胎儿在妊娠晚期（180,210和260天）的三个阶段进行了半腱肌的蛋白质组学分析。这些结果完善了对代谢和收缩途径中蛋白质谱的重要变化的认识。它们提供了关于诸如醛脱氢酶家族、烯醇化酶、二氢脂酰脱氢酶、肌钙蛋白T或肌球蛋白轻链异构体等蛋白质的新见解。这些数据具有农学上的应用，不仅对牛肉感官质量的管理，而且在医学背景下，牛的肌肉发生似乎非常类似于人类的一个。
Contractile and metabolic properties of bovine muscles play an important role in meat sensorial quality, particularly tenderness. Earlier studies based on Myosin heavy chain isoforms analyses and measurements of glycolytic and oxidative enzyme activities have demonstrated that the third trimester of foetal life in bovine is characterized by contractile and metabolic differentiation. In order to complete this data and to obtain a precise view of this phase and its regulation, we performed a proteomic analysis of Semitendinosus muscle from Charolais foetuses analysed at three stages of the third trimester of gestation (180, 210 and 260 days). The results complete the knowledge of important changes in the profiles of proteins from metabolic and contractile pathways. They provide new insights about proteins such as Aldehyde dehydrogenase family, Enolase, Dihydrolipoyl dehydrogenase, Troponin T or Myosin light chains isoforms. These data have agronomical applications not only for the management of beef sensorial quality but also in medical context, as bovine myogenesis appears very similar to human one.