Interaction of tick/borne encephalitis virus with mouse peritoneal macrophages. The effect of antiviral antibody and lectin.
Interaction of tick/borne encephalitis virus with mouse peritoneal macrophages. The effect of antiviral antibody and lectin.
复制标题
蜱/传播脑炎病毒与小鼠腹膜巨噬细胞的相互作用。
作者：
J. Kopecký;L. Grubhoffer;E. Tomková
体外实验观察了森林脑炎病毒Hypr株与小鼠巨噬细胞的相互作用。幼年鼠(8-11日龄)的巨噬细胞比成年鼠(25-30日龄)的巨噬细胞对病毒感染的容许性更强。亚中和剂量的抗TBE抗体增加了感染巨噬细胞的数量以及病毒向培养基中的释放。结合病毒和巨噬细胞表面的刀豆蛋白A(ConA)可增加病毒的摄取，但不能增加感染细胞的数量，也不能增加病毒向培养液中的释放。抗体和凝集素可改变TBE病毒与巨噬细胞的相互作用。然而，Fc受体介导的内吞作用似乎是增强配体作用的必要前提。
The interaction between tick-borne encephalitis (TBE) virus strain Hypr and mouse peritoneal macrophages was followed in vitro. Macrophages from juvenile mice (8-11 days old) were more permissive for virus infection than macrophages from adults (25-30 days old). Anti-TBE antibody in the subneutralizing dose increased the number of infected macrophages as well as virus release into the culture medium. Concanavalin A (con A), which bound to the virus as well as to the surface of macrophages, increased the uptake of the virus, but it neither enhanced the number of infected cells nor increased the virus release into medium. Antibody and lectin can modify the interaction between TBE virus and macrophages. Nevertheless, the Fc receptor-mediated endocytosis seems to be a necessary prerequisite for enhancing the effect of a ligand.