Electroacupuncture (EA) Speeds Up the Regulation of Hypothalamic Pituitary Adrenal Axis Dysfunction in Acute Surgical Trauma Rats: Mediated by Hypothalamic Gamma-Aminobutyric Acid (GABA) A Receptors
Electroacupuncture (EA) Speeds Up the Regulation of Hypothalamic Pituitary Adrenal Axis Dysfunction in Acute Surgical Trauma Rats: Mediated by Hypothalamic Gamma-Aminobutyric Acid (GABA) A Receptors
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DOI：
10.4236/jbbs.2018.812042
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发表时间：
2018-12
期刊：
影响因子：
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通讯作者：
Liting Zhu;Jing Zhu;Zhejun Chen;Zehui Meng;M. Ju;Mizhen Zhang;Gencheng Wu;Zhanzhuang Tian
中科院分区：
文献类型：
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作者：
Liting Zhu;Jing Zhu;Zhejun Chen;Zehui Meng;M. Ju;Mizhen Zhang;Gencheng Wu;Zhanzhuang Tian
下丘脑促肾上腺皮质激素释放因子（CRF）在手术创伤诱导的应激反应中直接激活下丘脑垂体肾上腺轴（HPA轴）。电针已被证明在乳腺手术、结直肠手术、直肠切除术和开颅手术中具有缓解压力的作用。本研究旨在探讨电针对手术创伤大鼠下丘脑CRF的调节作用。实验一：将SD雄性大鼠随机分为正常组、模型组（10%肝部分切除）、假电针组和电针组。电针组和假电针组分别于术前24 h和术后即刻刺激ST_（36）-足三里、SP_（6）-三阴交穴2次。分别于术后2、4、8、24 h采用放射免疫法（RIA）、免疫印迹法（Western blot）、实时荧光定量PCR（real-time PCR）和免疫组化法检测下丘脑CRF、CRFR、GABA受体、谷氨酸脱羧酶（GAD）、血清促肾上腺皮质激素（ACTH）和皮质酮（CORT）的表达。实验二将SD雄性大鼠分为正常组、模型组、模型+溶媒组、模型+ L-838，417电针组和电针+L-838，417电针组。结果表明：模型组下丘脑CRF、血清ACTH、CORT水平较模型组升高，电针组较模型组降低。与模型组相比，电针组下丘脑氨基丁酸（GABA）受体Aα3 mRNA和蛋白表达明显升高。结论：电针可通过促进下丘脑GABA的合成，从而增强GABA受体对急性手术创伤大鼠HPA轴功能障碍的抑制性调节，减轻手术应激反应。
Hypothalamic Corticotropin-releasing factor (CRF) directly activates the hypothalamic pituitary adrenal axis (HPA axis) during the surgical trauma induced stress response. Electroacupuncture (EA) has been demonstrated to have stress relieving effects in breast surgery, colorectal surgery, prostatectomy and craniotomy. This study was aimed to investigate the hypothesis that EA could regulate hypothalamic CRF in surgical trauma rats. In experiment one, Sprague-Dawley (SD) male rats were divided into intact, model (10% partial hepatectomy), sham EA and EA group. Rats from the Sham EA and EA group were stimulated at ST36-Zusanli and SP6-Sanyiniiao acupoints twice, 24 hours before the surgery and immediately after the surgery. Expressions of hypothalamic CRF and CRFR, GABA receptors, glutamate decarboxylase (GAD), serum adrenocorticotropic hormone (ACTH) and Corticosterone (CORT) were observed at 2, 4, 8 and 24 h after the surgery by radioimmunoassay (RIA), western blot, real-time PCR and immunohistochemistry. In the experiment two, SD male rats were divided into the intact, model, model + vehicle, model + L-838,417 EA and EA + L838,417 group. It was found that hypothalamus CRF, serum ACTH and CORT levels were increased in model group compared with the intact group, and those in the EA group decreased in comparison with the model group. Compared with the model group, hypothalamus-aminobutyric acid (GABA) receptor Aα3 mRNA and protein expressions of the EA group raised strikingly. In conclusion, EA alleviated surgical stress response by improving the GABA synthesis in hypothalamus, thus enhancing GABA receptors’ inhibitory regulation of the HPA axis dysfunction in rats with acute surgical trauma.