Evolutionarily conserved role for SoxC genes in neural crest specification and neuronal differentiation.
Evolutionarily conserved role for SoxC genes in neural crest specification and neuronal differentiation.
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DOI：
10.1016/j.ydbio.2014.09.022
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发表时间：
2015-01-15
影响因子：
2.7
通讯作者：
Bronner ME
中科院分区：
文献类型：
--
作者：
Uy BR;Simoes-Costa M;Koo DE;Sauka-Spengler T;Bronner ME
Sox转录因子家族的成员在脊椎动物和无脊椎动物中发挥着各种重要的发育作用。虽然SoxBs和SoxEs分别参与神经和神经嵴的发育，但对SoxC亚家族成员的了解要少得多。为了解决这一问题，从进化的角度来看，我们比较SoxC基因的表达和功能的神经嵴细胞及其衍生物七鳃鳗（Petromyzon marinus），基础脊椎动物，青蛙（非洲爪蟾）。转录本分布分析揭示了七鳃鳗和X.在迁移前神经嵴、鳃弓和颅神经节中的laevis SoxC表达。此外，吗啉介导的功能丧失的选择SoxC家族成员在两种生物体的神经嵴发育方面表现出重要作用。结果表明，重要的和保守的功能SoxC基因在脊椎动物的进化和一个特别关键的，以前未被认识到的作用，在早期神经嵴规范。
Members of the Sox family of transcription factors play a variety of critical developmental roles in both vertebrates and invertebrates. Whereas SoxBs and SoxEs are involved in neural and neural crest development, respectively, far less is known about members of the SoxC subfamily. To address this from an evolutionary perspective, we compare expression and function of SoxC genes in neural crest cells and their derivatives in lamprey (Petromyzon marinus), a basal vertebrate, to frog (Xenopus laevis). Analysis of transcript distribution from reveals conservation of lamprey and X. laevis SoxC expression in premigratory neural crest, branchial arches, and cranial ganglia. Moreover, morpholino-mediated loss-of-function of selected SoxC family members demonstrates essential roles in aspects of neural crest development in both organisms. The results suggest important and conserved functions of SoxC genes during vertebrate evolution and a particularly critical, previously unrecognized role in early neural crest specification.