Arabidopsis thaliana miRNAs promote embryo pattern formation beginning in the zygote
Arabidopsis thaliana miRNAs promote embryo pattern formation beginning in the zygote
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DOI：
10.1016/j.ydbio.2017.09.009
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发表时间：
2017-11-15
影响因子：
2.7
通讯作者：
Gillmor, C. Stewart
中科院分区：
文献类型：
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作者：
Armenta-Medina, Alma;Lepe-Soltero, Daniel;Gillmor, C. Stewart
mirna是细胞身份的重要调节因子，但它们在植物早期胚胎发育中的作用仍未得到充分研究。为了确定miRNA在胚胎发生中促进模式形成的最早阶段，我们检测了拟南芥miRNA生物发生酶DICER-LIKE 1 （DCL1）、SERRATE （SE）和HYPONASTIC LEAVES 1 （HYL1）的一系列突变等位基因。从合子到胚胎发生的球形阶段，戴尔、se和hyl1胚胎都存在细胞缺陷和图案缺陷。为了鉴定在早期胚胎发育中表达的miRNA，我们对来自球形期哥伦比亚野生型（wt）和se-1胚胎的mrna进行了测序，并鉴定了可能对应100个miRNA前体的转录本。考虑到wt和se-1之间的基因组位置和转录增加，这些mirna中有39个被预测为真正的早期胚胎mirna。其中包括miR156、miR159、miR160、miR161、miR164、miR165、miR166、miR167、miR168、miR171、miR319、miR390和miR394等保守mirna，以及功能尚未被鉴定的mirna。我们的分析表明，miRNAs从合子开始促进模式形成，并为拟南芥胚胎发生中单个miRNAs的功能研究提供了一个全面的数据集。
miRNAs are essential regulators of cell identity, yet their role in early embryo development in plants remains largely unexplored. To determine the earliest stage at which miRNAs act to promote pattern formation in embryogenesis, we examined a series of mutant alleles in the Arabidopsis thaliana miRNA biogenesis enzymes DICER-LIKE 1 (DCL1), SERRATE (SE), and HYPONASTIC LEAVES 1 (HYL1). Cellular and patterning defects were observed in dell, se and hyl1 embryos from the zygote through the globular stage of embryogenesis. To identify miRNAs that are expressed in early embryogenesis, we sequenced mRNAs from globular stage Columbia wild type (wt) and se-1 embryos, and identified transcripts potentially corresponding to 100 miRNA precursors. Considering genome location and transcript increase between wt and se-1, 39 of these MIRNAs are predicted to be bona fide early embryo miRNAs. Among these are conserved miRNAs such as miR156, miR159, miR160, miR161, miR164, miR165, miR166, miR167, miR168, miR171, miR319, miR390 and miR394, as well as miRNAs whose function has never been characterized. Our analysis demonstrates that miRNAs promote pattern formation beginning in the zygote, and provides a comprehensive dataset for functional studies of individual miRNAs in Arabidopsis embryogenesis.