Performance of Gram staining on blood cultures flagged negative by an automated blood culture system
Performance of Gram staining on blood cultures flagged negative by an automated blood culture system
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DOI：
10.1007/s10096-015-2383-0
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发表时间：
2015-08-01
影响因子：
4.5
通讯作者：
Brodsky, D.
中科院分区：
文献类型：
--
作者：
Peretz, A.;Isakovich, N.;Brodsky, D.
血液是送到微生物实验室进行培养的最重要的标本之一。大多数血培养培养时间为5-7天，除非怀疑感染是由增殖缓慢的微生物引起的，或血液中有少量细菌表达的感染。因此，在孵育结束时，阳性培养物的错误识别和假阴性结果是一个真实的可能性。这项工作的目的是通过革兰氏染色确认在培养结束时由BACTEC(TM) FX系统鉴定为阴性的血液培养物中缺乏任何微生物。所有经BACTEC FX系统定义为阴性的瓶均使用自动设备进行革兰氏染色，并接种于固体生长培养基上。在我们的工作中，在培养结束时被BACTEC FX系统定义为阴性的15个培养物在革兰氏染色时发现含有微生物。在阴性培养瓶中生长的大多数细菌和真菌的主要特征是生长缓慢。这一发现提出了一个有问题的问题，即需要对所有血液培养物进行革兰氏染色，这可能使常规实验室工作超负荷，特别是为大型医疗中心服务并接受大量血液培养物的实验室。
Blood is one of the most important specimens sent to a microbiology laboratory for culture. Most blood cultures are incubated for 5-7 days, except in cases where there is a suspicion of infection caused by microorganisms that proliferate slowly, or infections expressed by a small number of bacteria in the bloodstream. Therefore, at the end of incubation, misidentification of positive cultures and false-negative results are a real possibility. The aim of this work was to perform a confirmation by Gram staining of the lack of any microorganisms in blood cultures that were identified as negative by the BACTEC(TM) FX system at the end of incubation. All bottles defined as negative by the BACTEC FX system were Gram-stained using an automatic device and inoculated on solid growth media. In our work, 15 cultures that were defined as negative by the BACTEC FX system at the end of the incubation were found to contain microorganisms when Gram-stained. The main characteristic of most bacteria and fungi growing in the culture bottles that were defined as negative was slow growth. This finding raises a problematic issue concerning the need to perform Gram staining of all blood cultures, which could overload the routine laboratory work, especially laboratories serving large medical centers and receiving a large number of blood cultures.