Spatiotemporal regulation of the Pak1 kinase
Spatiotemporal regulation of the Pak1 kinase
复制标题
DOI：
10.1042/bst0330646
复制
发表时间：
2005-08-01
影响因子：
3.9
通讯作者：
de Gunzburg, J
中科院分区：
文献类型：
--
作者：
Parrini, MC;Matsuda, M;de Gunzburg, J
Pak 1（p21激活激酶1）是肌动蛋白细胞骨架、粘附和细胞运动的关键调节因子。这种生物学作用需要对其定位和活性进行严格的空间和动力学控制。我们在这里总结目前的知识Pak 1在体内的动力学。无活性的二聚体Pak 1主要存在于胞质中。与激活剂Cdc 42-GTP和Rac 1-GTP的局部相互作用刺激细胞突起部位的激酶。此外，Pak 1动态地参与多蛋白复合物，形成与细胞外基质的粘附。活细胞上的尖端显微镜技术终于揭示了调节Pak 1的复杂时空机制。
Pak1 (p21-activated kinase 1) is a key regulator of the actin cytoskeleton, adhesion and cell motility. Such biological roles require a tight spatial and kinetic control of its localization and activity. We summarize here the current knowledge on Pak1 dynamics in vivo. Inactive dimeric Pak1 is mainly cytosolic. Localized interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions. Moreover, Pak1 is dynamically engaged into multiprotein complexes forming adhesions to the extracellular matrix. Cutting edge microscopy technologies on living cells are finally shedding light on the intricate spatiotemporal mechanisms regulating Pak1.