Development of SSR molecular markers for Allium mongolicum
Development of SSR molecular markers for Allium mongolicum
复制标题
沙葱SSR分子标记的研制
DOI：
10.1007/s13258-017-0601-0
复制
发表时间：
2017-12-01
期刊：
影响因子：
2.1
通讯作者：
Yu, Qiushi
中科院分区：
文献类型：
--
作者：
Hu, Jing;Hu, Xiaoke;Yu, Qiushi
蒙古葱是一种食味、营养、药用价值高、抗旱的野生植物。然而，由于分子标记的缺乏，导致分子标记数据、群体遗传结构和遗传育种准确性等一系列遗传问题仍然是未知的。在本研究中，共获得了1,409,706个经过高质量过滤和修剪的蒙古包个体归一化基因组DNA读数。这些序列被组装成2,093,593个高质量的contigs，共筛选到219个简单的序列重复位点。选择其中60个来验证扩增并确定基因组DNA池中的多态性程度。15对引物成功扩增DNA片段，并检测到大量多态性。在10个不同地理位置的蒙古冬青群体中，等位基因数量在3 ~ 8个之间。观察到的杂合度为0.350 ~ 0.860，期望杂合度为0.516 ~ 0.931。本研究结果为蒙古沙乌属植物的研究提供了宝贵的资源。
Allium mongolicum is high palatability, nutrient, medicinal value and drought resistance wild plant. However, a lack of molecular markers of this plant results in a series of genetic questions remain largely unknown, including molecular marker data, population genetic structure and accuracy of genetic breeding. In this study, a total of 1,409,706 quality-filtered and trimmed reads were obtained from the normalized genomic DNA of pooled A. mongolicum individuals. These sequences were assembled into 2,093,593 high quality contigs, and a total of 219 simple sequence repeats loci were screened. Sixty of them were selected to validate amplification and to determine the degree of polymorphism in the genomic DNA pools. Fifteen primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism. The number of alleles in ten geographically diverse A. mongolicum populations ranged from 3 to 8 per locus. The observed and expected heterozygosities ranged from 0.350 to 0.860 and 0.516 to 0.931, respectively. Our results provide a valuable resource for A. mongolicum research.