Thiolene- and Polycaprolactone Methacrylate-Based Polymerized High Internal Phase Emulsion (PolyHIPE) Scaffolds for Tissue Engineering
Thiolene- and Polycaprolactone Methacrylate-Based Polymerized High Internal Phase Emulsion (PolyHIPE) Scaffolds for Tissue Engineering
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DOI：
10.1021/acs.biomac.1c01129
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发表时间：
2022-03-14
影响因子：
6.2
通讯作者：
Claeyssens, Frederik
中科院分区：
文献类型：
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作者：
Dikici, Betul Aldemir;Malayeri, Atra;Claeyssens, Frederik
高度多孔的乳液模板聚合物（PolyHIPE）在组织工程和再生医学支架的制造中提供了许多潜在的优势。多孔性使得细胞向内生长和营养物在支架内扩散，以及废物从支架移除。乳液模板单独提供的特性包括提供高互连孔隙率，以及与增材制造相结合，将受控多尺度孔隙率引入复杂或定制结构的机会。然而，报道的用于PolyHIPE制备的大多数单体系统不适合临床应用，因为它们是不可降解的。硫醇-烯化学是生产可生物降解的光固化PolyHIPE的一种有前途的途径，用于使用常规或增材制造方法制造支架;然而，关于这种方法的研究相对较少。本研究报告了通过光引发的硫醇烯点击反应制备基于硫醇和聚己内酯（PCL）的PolyHIPE材料的基础。在PolyHIPE制剂中使用两种不同的制剂，三臂PCL甲基丙烯酸酯（3 PCLMA）或四臂PCL甲基丙烯酸酯（4PCLMA）部分。使用人皮肤成纤维细胞（HDF）和人骨肉瘤细胞系（MG-63）通过DNA定量测定研究了PolyHIPE的生物相容性，并且开发的PolyHIPE显示出能够支持细胞附着和活力。
Highly porous emulsion templated polymers (PolyHIPEs) provide a number of potential advantages in the fabrication of scaffolds for tissue engineering and regenerative medicine. Porosity enables cell ingrowth and nutrient diffusion within, as well as waste removal from, the scaffold. The properties offered by emulsion templating alone include the provision of high interconnected porosity, and, in combination with additive manufacturing, the opportunity to introduce controlled multiscale porosity to complex or custom structures. However, the majority of monomer systems reported for PolyHIPE preparation are unsuitable for clinical applications as they are nondegradable. Thiol-ene chemistry is a promising route to produce biodegradable photocurable PolyHIPEs for the fabrication of scaffolds using conventional or additive manufacturing methods; however, relatively little research has been reported on this approach. This study reports the groundwork to fabricate thiol- and polycaprolactone (PCL)-based PolyHIPE materials via a photoinitiated thiolene click reaction. Two different formulations, either three-arm PCL methacrylate (3PCLMA) or four-arm PCL methacrylate (4PCLMA) moieties, were used in the PolyHIPE formulation. Biocompatibility of the PolyHIPEs was investigated using human dermal fibroblasts (HDFs) and human osteosarcoma cell line (MG-63) by DNA quantification assay, and developed PolyHIPEs were shown to be capable of supporting cell attachment and viability.