Ca analysis: an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis.

Ca analysis: an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis.
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DOI:
10.1016/j.cmpb.2013.09.004
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发表时间:
2014
影响因子:
6.1
通讯作者:
Greensmith DJ
Greensmith DJ
中科院分区:
工程技术2区
文献类型:
--
作者:
Greensmith DJ

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在这里,我提出了一个基于Excel的程序,用于使用荧光指示剂记录的细胞内钙瞬变的分析。该程序可以执行所有必要的步骤,将记录的原始电压变化转换为有意义的生理信息。该程序执行两个基本过程。(1)它可以通过几种方法准备原始信号。(2)然后,它可以用于分析准备的数据,以提供信息,如绝对细胞内钙水平。此外,Ca的变化率可以使用多重同时回归分析来测量。我证明了这个程序的性能与商用软件一样好,但具有许多优点,即创建一个简化的,独立的分析工作流程。
Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological information. The program performs two fundamental processes. (1) It can prepare the raw signal by several methods. (2) It can then be used to analyze the prepared data to provide information such as absolute intracellular Ca levels. Also, the rates of change of Ca can be measured using multiple, simultaneous regression analysis. I demonstrate that this program performs equally well as commercially available software, but has numerous advantages, namely creating a simplified, self-contained analysis workflow.
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