Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the proteins.
Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the proteins.
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DOI：
10.1186/gb-2005-6-7-r56
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发表时间：
2005
期刊：
影响因子：
12.3
通讯作者：
Thornton JM
中科院分区：
文献类型：
--
作者：
Freilich S;Massingham T;Bhattacharyya S;Ponsting H;Lyons PA;Freeman TC;Thornton JM
小鼠基因表达的微阵列分析结合蛋白质的功能和系统分类表明，系统年龄（而不是功能）是形成蛋白质表达谱的主导因素。完整的基因组序列信息与表达数据的结合使我们能够表征蛋白质的进化起源或功能类别与其表达模式之间的关系。在这项研究中，小鼠蛋白质被分配到功能和系统组和不同的蛋白质组的基因表达模式进行了研究，在各种小鼠组织的微阵列分析。我们的研究结果表明，普遍分布在所有组织中的蛋白质主要是酶和转运蛋白。相比之下，组织特异性集由调节蛋白（信号转导和转录因子）主导。观察到后生动物特异性蛋白质的组织特异性增加的趋势。由于系统群的组成与功能群的组成高度相关，因此对数据进行了测试，以确定功能或系统年龄这两个因素中的哪一个在形成蛋白质的表达谱中占主导地位。发现功能群之间基因表达模式的差异主要反映了它们不同的种系起源。组织特异性和系统年龄之间的联系不能用最近的进化速度来解释。最后，虽然后生动物特异性蛋白质往往是组织特异性的物种保守的蛋白质相比，存在于所有领域的生活，许多这样的“通用”蛋白质也是组织特异性的。后生动物细胞的最小细胞转录组不同于原始单细胞真核生物：增加了新的功能（后生动物特异性蛋白质），而其他功能变得专门化，不再发生在所有细胞（组织特异性前后生动物蛋白质）。
A microaaray analysis of mouse gene expression combined with the proteins functional and phyletic classification suggests that phyletic age (and not function) is the dominant factor shaping the expression profle of a protein. The combination of complete genome sequence information with expression data enables us to characterize the relationship between a protein's evolutionary origin or functional category and its expression pattern. In this study, mouse proteins were assigned into functional and phyletic groups and the gene expression patterns of the different protein groupings were examined by microarray analysis in various mouse tissues. Our results suggest that the proteins that are universally distributed in all tissues are predominantly enzymes and transporters. In contrast, the tissue-specific set is dominated by regulatory proteins (signal transduction and transcription factors). An increased tendency to tissue-specificity is observed for metazoan-specific proteins. As the composition of the phyletic groups highly correlates with that of the functional groups, the data were tested in order to determine which of the two factors - function or phyletic age - is dominant in shaping the expression profile of a protein. The observed differences in expression patterns of genes between functional groups were found mainly to reflect their different phyletic origin. The connection between tissue specificity and phyletic age cannot be explained by the recent rate of evolution. Finally, although metazoan-specific proteins tend to be tissue-specific compared with phyletically conserved proteins present in all domains of life, many such 'universal' proteins are also tissue-specific. The minimal cellular transcriptome of the metazoan cell differs from that of the ancestral unicellular eukaryote: new functions were added (metazoan-specific proteins), whilst other functions became specialized and no longer took place in all cells (tissue-specific pre-metazoan proteins).