A novel wheat cysteine-rich receptor-like kinase gene CRK41 is involved in the regulation of seed germination under osmotic stress in Arabidopsis thaliana
A novel wheat cysteine-rich receptor-like kinase gene CRK41 is involved in the regulation of seed germination under osmotic stress in Arabidopsis thaliana
复制标题
一种新型小麦富含半胱氨酸受体样激酶基因CRK41参与拟南芥渗透胁迫下种子萌发的调节
DOI：
10.1007/s12374-017-0248-z
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发表时间：
2017-12-01
影响因子：
2.9
通讯作者：
Wang, Mei
中科院分区：
文献类型：
--
作者：
Chen, Donghua;Wu, Jing;Wang, Mei
面包小麦基因TaCRK41编码富含半胱氨酸的受体激酶。它是下调各种非生物胁迫和暴露于脱落酸。在拟南芥原生质体中的瞬时表达实验包括编码绿色荧光蛋白的序列与来自耐盐小麦品种陕荣3号（SR 3）或其盐敏感祖先品种济南177（JN177）的TaCRK 41等位基因之间的融合，表明TaCRK 41产物沉积在细胞质中。来源于SR3和JN177的重组TaCRK41在体外没有显示激酶活性。TaCRK41在A.结果表明，在种子萌发过程中，拟南芥对外源脱落酸和盐的敏感性均显著降低。ABA诱导的ABI3、ABI5和ABI5控制的基因EM1和EM6的转录水平在受到胁迫的植物中显著降低。
The bread wheat gene TaCRK41 encodes a cysteine-rich receptor kinase. It is down-regulated by various abiotic stresses and by exposure to abscisic acid. A transient expression experiment in Arabidopsis thaliana protoplasts involving a fusion between the sequence encoding green fluorescence protein and the TaCRK41 allele from either the salinity tolerant bread wheat cultivar Shanrong No. 3 (SR3) or its salinity sensitive progenitor cultivar Jinan 177 (JN177) showed that the TaCRK41 product is deposited in the cytoplasm. Recombinant TaCRK41 originating from both SR3 and JN177 displayed no kinase activity in vitro. The constitutive expression of TaCRK41 in A. thaliana resulted in a marked reduction in the plants’ sensitivity to both exogenous abscisic acid and to salinity during germination. The level of ABA-induced transcription of ABI3, ABI5 and the ABI5-controlled genes EM1 and EM6 was significantly reduced in plants subjected to stress.