Expression and localization of the transcription factor JunD in the duct system of mouse submandibular gland
Expression and localization of the transcription factor JunD in the duct system of mouse submandibular gland
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DOI：
10.1177/002215540405200406
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发表时间：
2004-04-01
影响因子：
3.2
通讯作者：
Iseki, S
中科院分区：
文献类型：
--
作者：
Hipkaeo, W;Wakayama, T;Iseki, S
我们通过免疫印迹和免疫组织化学研究了 JunD（转录因子激活蛋白-1 (AP-1) 的一个组成部分）在小鼠颌下腺中的表达和定位。在成年小鼠中，构成 AP-1 复合体的所有 7 个 Jun 和 Fos 家族成员在雌性腺体中的表达量均高于雄性腺体，其中 JunD 是其中最丰富的成员。 JunD 的免疫反应性仅位于腺体的导管系统中，其中它位于闰管 (ID) 细胞的细胞核和位于 ID 附近的横纹管 (SD) 细胞亚群。相比之下，颗粒曲管 (GCT) 细胞；男性腺体中含量丰富得多，但缺乏 JunD。在男性腺体产后发育过程中，JunD 从导管细胞中丢失，因为它们在产后 3-5 周分化为 GCT 细胞。当通过给予睾酮在成年女性腺体中诱导GCT分化时，许多JunD阴性SID细胞在6-24小时后被暂时诱导表达JunD，但这些细胞在48小时完全转化为GCT细胞时失去了JunD。这些结果表明JunD参与了小鼠颌下腺导管系统的分化，其中雄激素/雄激素受体系统与AP-1复合物之间存在串扰。
We studied the expression and localization of JunD, a component of the transcription factor activator protein-1 (AP-1), in the mouse submanclibular gland with immunoblotting and immunohistochemistry. In adult mice, all seven Jun and Fos family members constituting the AP-1 complex were expressed more abundantly in the female gland than in the male gland, and JunD was the most abundant of the members. Immunoreactivity for JunD was localized exclusively in the duct system of the gland, in which it was localized to the nuclei of intercalated duct (ID) cells and a subpopulation of striated duct (SD) cells located adjacent to ID. In contrast, granular convoluted tubule (GCT) cells; which are much more abundant in the male gland, were devoid of JunD. During postnatal development of the male gland, JunD was lost from the duct cells as they differentiated to GCT cells at 3-5 weeks postpartum. When GCT differentiation was induced in adult female gland by testosterone administration, many JunD-negative SID cells were temporarily induced to express JunD after 6-24 hr, but those cells lost JunD as they completely converted to GCT cells by 48 hr. These results suggested that JunD is involved in the differentiation of the duct system of mouse submanclibular gland, in which there is crosstalk between the androgen/androgen receptor system and the AP-1 complex.