Overexpression of circFNDC3B promotes the progression of oral tongue squamous cell carcinoma through the miR-1322/MED1 axis
Overexpression of circFNDC3B promotes the progression of oral tongue squamous cell carcinoma through the miR-1322/MED1 axis
复制标题
circFNDC3B过表达通过miR-1322/MED1轴促进口腔舌鳞状细胞癌进展
DOI：
10.1002/hed.27152
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发表时间：
2022-08-02
影响因子：
2.9
通讯作者：
Liang，Jin
中科院分区：
文献类型：
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作者：
Chen，Xiao;Kong，Deyu;Liang，Jin
背景circFNDC3B在调节口腔舌鳞状细胞癌（OTSCC）发展中的潜在作用尚不清楚。方法检测环cfndc3b在OTSCC组织或细胞系中的表达水平，并分析其体外和体内功能。circFNDC3B、miR‐1322和MED1之间的相互作用通过荧光素酶报告基因和RNA下拉实验得到验证。结果circFNDC3B在OTSCC组织或细胞系中的表达水平高于对照组。siRNA介导的circFNDC3B抑制导致增殖、迁移和侵袭减弱，miR‐1322可逆转这一过程。在体外，OTSCC细胞中MED1的过表达部分逆转了si - circFNDC3B或miR - 1322模拟物的肿瘤抑制功能。circFNDC3B过表达可显著促进体内肿瘤生长。circFNDC3B直接与miR‐1322结合，从而促进了OTSCC细胞中MED1的表达。结论circFNDC3B/miR‐1322/MED1轴参与OTSCC的进展，可能为OTSCC提供新的治疗靶点。
BackgroundThe potential role of circFNDC3B in regulating oral tongue squamous cell carcinoma development (OTSCC) remains unknown.MethodsThe level of circFNDC3B in OTSCC tissues or cell lines was measured and its functionin vitroandin vivowas analyzed. Interactions among circFNDC3B, miR‐1322, and MED1 were verified by luciferase reporter and RNA pull‐down assays.ResultsThe level of circFNDC3B in tissues or cell lines of OTSCC was higher than that in control groups. siRNA‐mediated circFNDC3B inhibition resulted in weakened proliferation, migration, and invasion, which was reversed by miR‐1322. Overexpression of MED1 in OTSCC cells partially reversed the tumor suppression functions of si‐circFNDC3B or miR‐1322 mimicsin vitro. circFNDC3B overexpression dramatically promoted tumor growthin vivo. circFNDC3B directly bound with miR‐1322 and consequently promoted the MED1 expression in OTSCC cells.ConclusionsThe circFNDC3B/miR‐1322/MED1 axis participates in OTSCC progression, which may provide novel therapeutic targets for OTSCC.