Isolation and characterization of different plant associated bacteria and their potential to degrade polychlorinated biphenyls
Isolation and characterization of different plant associated bacteria and their potential to degrade polychlorinated biphenyls
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DOI：
10.1016/j.ibiod.2009.03.009
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发表时间：
2009-09-01
影响因子：
4.8
通讯作者：
Mackova, Martina
中科院分区：
文献类型：
--
作者：
Ionescu, Marieta;Beranova, Katarina;Mackova, Martina
在我们的实验中，评估了不同植物对微生物活动的影响，导致长期污染土壤中的降解和 PCB 去除。对种植在受 PCB 污染的土壤以及相同但无 PCB 植被的土壤中的烟草 (Nicotiana tabacum)、黑龙葵 (Solanum nigrum)、辣根 (Armoracia rusticana) 和山羊柳 (Salix caprea) 等个别植物物种的根际细菌总数和代表主要微生物区系的细菌进行了分离和生物化学表征。从根际土壤分离并在以联苯作为唯一碳源的基本培养基上预培养后，检测到单个植物根部分泌物刺激的多氯联苯细菌降解剂。使用预先设计的特异性引物，通过巢式PCR技术检测根际微生物基因组中的BphA1基因（细菌需氧PCB降解途径的第一个基因）。各植物根际含有不同的细菌种类，主要为假单胞菌属、农杆菌属、苍白杆菌属等革兰氏阴性非发酵菌。仅在其中几个基因组中发现了基因 BphA1。在测试的物种中，S. caprea 和 A. rusticana 已被证明是用于根部修复目的的有希望的候选者。 (C) 2009 年，爱思唯尔有限公司出版。
In our experiments the effect of different plants on microbial activities resulting in degradation and PCB removal from long-term contaminated soil was evaluated. Total bacteria and bacteria representing the dominating microflora within rhizosphere of individual plant species - tobacco (Nicotiana tabacum), black nightshade (Solanum nigrum), horseradish (Armoracia rusticana) and goat willow (Salix caprea) planted in PCB contaminated soil as well as from the same, but non-vegetated PCBs soil, were isolated and biochemically characterized. PCB bacterial degraders, stimulated by root exudates of individual plants, were detected after isolation from rhizosphere soil and precultivation on minimal medium with biphenyl as the sole carbon source. Detection of BphA1 gene (first gene of bacterial aerobic PCB degradative pathway) in genomes of rhizosphere microorganisms was performed by nested PCR technique using previously designed specific primers. Rhizosphere of individual plants contained different bacterial species, mostly gram-negative non-fermenting bacteria of Pseudomonas, Agrobacterium, Ochrobactrum and other species. Gene BphA1 was identified in genome of only several of them. From tested species, S. caprea and A. rusticana have shown to be promising candidates for rhizoremediation purposes. (C) 2009 Published by Elsevier Ltd.