Non-destructive visualization and quantification of roots using computed tomography
Non-destructive visualization and quantification of roots using computed tomography
复制标题
DOI：
10.1016/j.soilbio.2006.07.018
复制
发表时间：
2007-02-01
影响因子：
9.7
通讯作者：
Deadman, M.
中科院分区：
文献类型：
--
作者：
Perret, J. S.;Al-Belushi, M. E.;Deadman, M.
描述了一种使用计算机断层扫描（CT）和开发用于隔离和分析CT矩阵的计算机代码对相对较大的岩心进行根的非破坏性可视化和量化的协议。使用填充有已知几何形状和方向的不同土壤和材料（包括根段）的体模核心优化扫描仪设置。CT参数进行了优化（130 kV峰值电压和480 mAs），使用核心0.23 x 0.14 m直径。填充有以275 × 275 × 1000 μ m的体素分辨率扫描的单粒沙子。鹰嘴豆的根发芽后21天，如根的数量，它们的体积，长度，壁面积，曲折度和方向的定量属性，并与破坏性取样获得的结果进行比较。结果表明，与破坏性取样相比，CT方法系统性地低估了牙根长度（差异高达10%）。通过非破坏性算法估计的平均根段长度为28.1 mm，而通过破坏性分析估计的平均根段长度为36 mm。然而，非破坏性方法揭示了侵入性技术无法获得的细节。例如，牙根的平均弯曲度为2.5，表明其长度比其末端之间的距离大2.5。(c)2006年由Elsevier Ltd.出版
A protocol is described for non-destructive visualization and quantification of roots for relatively large core using computed tomography (CT) and computer codes developed to isolate and analyze the CT matrices. The scanner settings were optimized using a phantom core filled with different soil and materials (including root segments) of known geometry and orientation. CT parameters were optimized (130 kV peak voltage and 480 mAs), using a core 0.23 x 0.14 m diam. filled with a single grain sand scanned at a voxel resolution of 275 x 275 x 1000 mu m. Quantitative attributes of the roots of chickpea 21 days after germination such as the number of root laterals, their volume, length, wall area, tortuosity and orientation are presented and compared with results obtained by destructive sampling. Results suggest the CT approach systematically underestimated root length compared to destructive sampling (difference reaching up to 10%). The average root segment length estimated by the non-destructive algorithm was 28.1 mm compared to 36 mm by destructive analysis. However, the non-destructive approach revealed details that are not possible to obtain with invasive techniques. For instance, the root laterals had an average tortuosity of 2.5 indicating that their length was 2.5 greater than the distance between their extremities. (c) 2006 Published by Elsevier Ltd.