NEURAL AND HUMORAL-FACTORS SEPARATELY REGULATE NEUROPEPTIDE-Y, ENKEPHALIN, AND CHROMOGRANIN-A AND CHROMOGRANIN-B MESSENGER-RNA LEVELS IN RAT ADRENAL-MEDULLA
NEURAL AND HUMORAL-FACTORS SEPARATELY REGULATE NEUROPEPTIDE-Y, ENKEPHALIN, AND CHROMOGRANIN-A AND CHROMOGRANIN-B MESSENGER-RNA LEVELS IN RAT ADRENAL-MEDULLA
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DOI：
10.1073/pnas.85.9.3240
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发表时间：
1988-05-01
影响因子：
11.1
通讯作者：
EIDEN, LE
中科院分区：
文献类型：
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作者：
FISCHERCOLBRIE, R;IACANGELO, A;EIDEN, LE
在体研究了神经源性和体液性因素对大鼠肾上腺髓质几种分泌蛋白mRNA水平的影响。增加内脏神经活动产生（反射）与胰岛素治疗。注射胰岛素后24小时，编码神经肽（脑啡肽和神经肽Y）的mRNA水平增加了6.5倍，而主要分泌蛋白（嗜铬粒蛋白A和B）的mRNA水平没有变化。内脏神经的双侧横切完全阻止了这种增加。垂体切除术降低了嗜铬粒蛋白A的mRNA水平的32%的控制，这表明依赖于垂体-肾上腺轴的激素。用地塞米松治疗垂体切除的大鼠使嗜铬粒蛋白A mRNA恢复到基础水平。嗜铬粒蛋白B mRNA水平没有改变胰岛素治疗或垂体切除术。这些结果表明：（i）嗜铬颗粒中存在的不同类型的分泌蛋白受到不同机制的调节，（ii）这种调节发生在翻译前位点，（iii）嗜铬颗粒分泌成分的相对浓度可能会有所不同。分泌颗粒成分，这可能是重要的低血压或压力的改变组合物的意义进行了讨论。
The influence of neurogenic versus humoral factors on mRNA levels of several secretory proteins of rat adrenal medulla was studied in vivo. Increased splanchnic nerve activity was generated (reflexly) with insulin treatment. Twenty-four hours after insulin injection, levels of mRNAs encoding neuropeptides (enkephalin and neuropeptide Y) were increased 6.5-fold, wherease those of mRNAs for the major secretory proteins (chromogranins A and B) were unchanged. Bilateral transection of the splanchnic nerves completely prevented this increase. Hypophysectomy decreased levels of chromogranin A mRNA to 32% of control, suggesting a dependence on hormones of the pituitary-adrenal axis. Treatment of hypophysectomized rats with dexamethasone restored chromogranin A mRNA to basal levels. Chromogranin B mRNA levels were not changed by either insulin treatment or hypophysectomy. These results demonstrate (i) that different classes of secretory proteins present in chromaffin granules are regulated by different mechanisms, (ii) that this regulation occurs at a pretranslational site, and (iii) that the relative concentration of secretory constituents of chromaffin granules may vary. The significance of an altered composition of secretory-granule constituents, which may be important in hypotension or stress, is discussed.