Biological and biochemical characterization of a red-eye mutant in Nilaparvata lugens (Hemiptera: Delphacidae)
Biological and biochemical characterization of a red-eye mutant in Nilaparvata lugens (Hemiptera: Delphacidae)
复制标题
褐飞虱红眼突变体的生物学和生化特征（半翅目：飞虱科）
DOI：
10.1111/1744-7917.12049
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发表时间：
2014-08-01
期刊：
影响因子：
4
通讯作者：
Tang, Jian
中科院分区：
文献类型：
--
作者：
Liu, Shu-Hua;Yao, Jing;Tang, Jian
本实验室对亚洲主要水稻害虫褐飞虱（BPH）， Nilaparvata lugens （Stal）建立了红眼种群。除了红眼表型外，野生型（棕色眼）和突变型（红眼）在外部性状上没有其他差异。遗传分析表明，红眼表型由单个常染色体隐性等位基因控制。生物学研究发现，红眼突变群体的产蛋量和卵活力与野生型BPH没有显著差异。生化分析和电子显微镜检查显示，红眼突变体的黄瘤素（棕色）和蝶呤（红色）含量均降低，色素颗粒数量减少。因此，这两种色素的数量和比例的变化是这种红眼突变的生化基础。我们的研究结果表明，红眼突变基因（红色）可能参与了两种色素在色素运输、色素颗粒形成或其他过程中的一个共同基因位点。
A red-eye colony was established in our laboratory in brown planthopper (BPH), Nilaparvata lugens (Stal), a major rice pest in Asia. Except for the red-eye phenotype, no other differences were observed between the wild-type (brown eye) and the mutant-type (red eye) in external characters. Genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. Biological studies found that egg production and egg viability in the red-eye mutant colony were not significantly different from those in the wild-type BPH. Biochemical analysis and electronic microscopy examination revealed that the red-eye mutants contained decreased levels of both xanthommatin (brown) and pteridine (red) and reduced number of pigment granules. Thus, the changes of amount and ratio of the two pigments is the biochemical basis of this red-eye mutation. Our results indicate that the red-eye mutant gene (red) might be involved in one common gene locus shared by the two pigments in pigment transportation, pigment granule formation or some other processes.