Identification of a cell-surface protein involved in glucose assimilation and disruption of the crystalline region of cellulose by Cytophaga hutchinsonii
Identification of a cell-surface protein involved in glucose assimilation and disruption of the crystalline region of cellulose by Cytophaga hutchinsonii
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鉴定参与葡萄糖同化和哈钦森细胞噬菌体破坏纤维素结晶区的细胞表面蛋白
DOI：
10.1007/s10295-019-02212-3
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发表时间：
2019-07
影响因子：
3.4
通讯作者：
Lu Xuemei
中科院分区：
文献类型：
--
作者：
Wang Sen;Zhao Dong;Zhang Weican;Lu Xuemei
纤维素的结晶区是结晶纤维素利用的主要障碍。Cytophaga hutchinsonii通过一种未知的机制活跃地破坏纤维素的结晶区。进行转座子诱变以鉴定新的基因位点chu_1557，其是有效破坏纤维素的结晶区域所需的，并且CHU_1557的缺失导致葡萄糖同化效率降低。通过添加葡萄糖或在低浓度葡萄糖培养基中预培养，可以部分地保留突变体纤维素结晶区断裂的缺陷，从而提高其葡萄糖吸收效率。这些结果表明，胞外葡萄糖在C.哈钦森氏菌进一步的研究表明，在低葡萄糖浓度培养基中，外膜蛋白CHU_3732的表达因CHU_1557的缺失而下调。CHU_3732参与葡萄糖摄取，其表达由低浓度葡萄糖诱导。CHU_3732被预测为孔蛋白，因此我们推断它可能作为外膜中的葡萄糖转运通道。基于这些结果，我们推断CHU_1557在葡萄糖同化过程中发挥作用，其破坏影响其他与葡萄糖转运相关的蛋白质如CHU_3732的表达，进而影响细胞在低葡萄糖浓度培养基中的生长和纤维素结晶区的破坏。
The crystalline region of cellulose is the main barrier to the utilization of crystalline cellulose. Cytophaga hutchinsonii actively digests the crystalline region of cellulose by an unknown mechanism. Transposon mutagenesis was done to identify a novel gene locus chu_1557, which is required for efficient disruption of the crystalline region of cellulose, and the absence of CHU_1557 resulted in decreased glucose assimilation efficiency. The defect of the mutant in the disruption of the crystalline region of cellulose was partially retained by additional glucose or pre-culturing the mutant in a low glucose concentration medium which could improve its glucose absorption efficiency. These results suggested that extracellular glucose has important roles in the disruption of crystalline cellulose by C. hutchinsonii. Further study showed that the expression of an outer membrane protein CHU_3732 was downregulated by the absence of CHU_1557 in a low glucose concentration medium. CHU_3732 was involved in uptake of glucose and its expression was induced by a low concentration of glucose. CHU_3732 was predicted to be a porin, so we inferred that it may work as a glucose transport channel in the outer membrane. Based on these results, we deduced that CHU_1557 played a role in the process of glucose assimilation and its disruption affected the expression of other proteins related to glucose transportation such as CHU_3732, and then affected the cell growth in a low glucose concentration medium and disruption of the crystalline region of cellulose.
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影响因子：
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