A dual-modality optical coherence tomography and selective plane illumination microscopy system for mouse embryonic imaging.
A dual-modality optical coherence tomography and selective plane illumination microscopy system for mouse embryonic imaging.
复制标题
用于小鼠胚胎成像的双模态光学相干断层扫描和选择性平面照明显微镜系统。
DOI：
10.1109/embc.2017.8037742
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Larin,KirillV
中科院分区：
文献类型：
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作者：
ChenWu;ShihaoRan;Le,Henry;Singh,Manmohan;Larina,IrinaV;Mayerich,David;Dickinson,MaryE;Larin,KirillV
光学相干断层扫描（OCT）和选择性平面照明显微镜（SPIM）在小鼠胚胎研究中经常用于高分辨率三维成像。每种成像方法都具有独特而独立的优势：SPIM通过免疫荧光提供形态功能信息，OCT提供全胚胎3D成像方法。在这项研究中，我们将旋转成像OCT和SPIM结合成一个单一的双模态设备来成像E9.5小鼠胚胎。结果表明，双模态设置能够同时提供解剖和功能信息，以进行更全面的组织表征。
Both optical coherence tomography (OCT) and selective plane illumination microscopy (SPIM) are frequently used in mouse embryonic research for high-resolution three-dimensional imaging. Each of these imaging methods provide a unique and independent advantage: SPIM provides morpho-functional information through immunofluorescence and OCT provides a method for whole-embryo 3D imaging. In this study, we have combined rotational imaging OCT and SPIM into a single, dual-modality device to image E9.5 mouse embryos. The results demonstrate that the dual-modality setup is able to provide both anatomical and functional information simultaneously for more comprehensive tissue characterization.