Prevalence and genetic variability of Arcobacter species in mechanically separated turkey.
Prevalence and genetic variability of Arcobacter species in mechanically separated turkey.
复制标题
机械分离火鸡中弓形杆菌属的流行率和遗传变异性。
DOI：
10.4315/0362-028x-61.12.1623
复制
发表时间：
1998
影响因子：
2
通讯作者：
K. Harmon
中科院分区：
文献类型：
--
作者：
T. Manke;I. Wesley;J. Dickson;K. Harmon
1995年冬季和1996年夏秋两季对机械分离火鸡进行了弧菌属和布氏弧菌属调查。采用聚合酶链反应和种特异性寡核苷酸探针对Arcobacter spp和A. butzleri进行了鉴定。在395份机械分离的火鸡样品中，有77%（303份）分离出Arcobacter spp.，其中74%（223份）的火鸡样品中有buzleri杆菌阳性。在121株布氏单胞杆菌分离株中，86种不同的模式在扩增肠杆菌重复基因间一致序列后明显存在。遗传多态性的程度表明存在多种污染源。
A survey for Arcobacter spp. and Arcobacter butzleri in mechanically separated turkey was conducted during the winter of 1995 and summer and fall of 1996. Arcobacter spp. and A. butzleri were identified by polymerase chain reaction and species-specific oligonucleotide probes. Arcobacter spp. were isolated from 77% (303 out of 395) of the mechanically separated turkey samples with 74% (223 out of 303) of these samples positive for A. butzleri. Of the 121 A. butzleri isolates tested, 86 different patterns were evident following amplification of enterobacterial repetitive intergenic consensus sequences. The extent of genetic polymorphism indicated multiple sources of contamination.