Uc.206 regulates cell proliferation and apoptosis by targeting P53 in cervical cancer cells
Uc.206 regulates cell proliferation and apoptosis by targeting P53 in cervical cancer cells
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DOI：
10.4149/310_151017n538
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发表时间：
2016-01-01
期刊：
影响因子：
3
通讯作者：
Wang, C.
中科院分区：
文献类型：
--
作者：
Li, Q.;Li, X.;Wang, C.
超服务区(UCRs)是非蛋白质编码的基因序列，在不同物种之间具有严格的保守性。新的证据表明，编码非编码RNA(NcRNAs)的UCR是基因表达的调节因子。近几十年来，越来越多的证据表明UCRs参与了癌症的发生。以往的研究表明，uc.206的RNA在结直肠癌中的表达增加。到目前为止，uc.206在宫颈癌中的作用仍不明确。这项研究表明，uc.206在宫颈癌组织中显著上调，并与促凋亡基因P53在RNA水平的表达呈负相关。我们发现uc.206特异性地针对P53的3‘非翻译区(3’UTR)并调节其表达。抑制uc.206有效地延缓了宫颈细胞的增殖，促进了细胞的凋亡，并伴随着P53蛋白的表达增加。因此，这些发现提示uc.206作为一个新的癌基因，通过靶向p53基因，促进CC细胞的生长，这可能有利于宫颈癌的治疗。
Ultraconserved regions (UCRs) are non-protein coding gene sequences with strict conserved across among different species. Emerging evidence demonstrates that UCRs encoding noncoding RNAs (ncRNAs) serve as regulators of gene expression. In recent decades, increasing evidence implicates the involvement of UCRs in carcinogenesis. Previous studies showed RNA expression of uc.206 was increased in colorectal cancer. Until now, the role of uc.206 in cervical cancers remains undefined. This study revealed that uc.206 is significantly up-regulated in cervical cancer (CC) tissue and negatively correlates with the expression of the pro-apoptotic gene P53 in RNA level. We show that uc.206 specifically targets the 3' untranslated region (3'UTR) of P53 and regulates its expression. Inhibition of uc.206 effectively delays cervical cells proliferation and promotes apoptosis, accompanied by increased expression of P53 protein. Thus, these findings suggested that uc.206 acts as a novel oncogene by targeting the P53 gene and promoting CC cell growth, which might be beneficial for cervical cancer therapy.