MORPHOLOGY OF TYROSINE HYDROXYLASE-IMMUNOREACTIVE NEURONS IN THE HUMAN CEREBRAL-CORTEX
MORPHOLOGY OF TYROSINE HYDROXYLASE-IMMUNOREACTIVE NEURONS IN THE HUMAN CEREBRAL-CORTEX
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DOI：
10.1007/bf00253618
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发表时间：
1989-01-01
影响因子：
2
通讯作者：
DETRIBOLET, N
中科院分区：
文献类型：
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作者：
HORNUNG, JP;TORK, I;DETRIBOLET, N
在新鲜固定活检的人大脑皮层手术获得，免疫细胞化学染色与酪氨酸羟化酶（速率限制生物合成酶的儿茶酚胺）的抗体显示，除了致密的轴突丛，一个人口的免疫反应性细胞体。这些细胞的神经元性质通过以下方式确定：i）细胞体中存在丰富的粗面内质网，细胞体和树突上存在突触酶，以及ii）证明酪氨酸羟化酶免疫反应性细胞中缺乏与星形胶质细胞标志物胶质细胞酸性蛋白的反应性。酪氨酸羟化酶免疫反应阳性神经元被发现在所有领域的皮质采样，几乎只位于颗粒下层。大多数酪氨酸羟化酶免疫反应阳性细胞是双极和垂直取向，但少数有一个多极或水平的树突状乔木。这些细胞的树突是静脉曲张和刺，轴突非常薄。酪氨酸羟化酶免疫反应阳性神经元被报道短暂存在于发育中的哺乳动物大脑皮层，最近才在成熟的哺乳动物大脑皮层。人大脑皮层中含有儿茶酚胺合成酶的神经元将是重要的，特别是考虑到儿茶酚胺可能与一些主要的精神障碍有关。
In freshly fixed biopsies of human cerebral cortex obtained at surgery, immunocytochemical staining with antibodies against tyrosine hydroxylase (the rate limiting biosynthetic enzyme for catecholamines) revealed, in addition to a dense axonal plexus, a population of immunoreactive cell bodies. The neuronal nature of these cells was ascertained by: i) the presence of a rich rough endoplasmic reticulum in the cell body and of synapases on the cell body and dendrites, and ii) the demonstration of the lack of reactivity with the astroglial marker, glial fibrillary acidic protein, in the tyrosine hydroxylase-immunoreactive cells. The tyrosine hydroxylase-immunoreactive neurons were found in all areas of cortex sampled, and were located almost exclusively in the infragranular layers. Most tyrosine hydroxylase-immunoreactive cells were bipolar and were vertically oriented, but a few had a multipolar or horizontal dendritic arbor. The dendrites of these cells were varicose and aspiny, and the axons were very thin. Tyrosine hydroxylase-immunoreactive neurons were reported to be present transiently in the developing mammalian cerebral cortex and only recently in cerebral cortex of mature mammalian brains. Internuncial neurons in the human cerebral cortex containing a catecholamine synthesizing enzyme would be significant, in particular considering that catecholamines are likely to be involved in some major mental disorders.