An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation
An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation
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DOI：
10.1039/c9cc08664a
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发表时间：
2020-03-28
影响因子：
4.9
通讯作者：
Watanabe, Tomonobu M.
中科院分区：
文献类型：
--
作者：
Kakizuka, Taishi;Takai, Akira;Watanabe, Tomonobu M.
基于荧光蛋白的报告系统用于跟踪细胞中的基因表达。在这里，我们提出了一种改进的生物发光共振能量转移（BRET）报告器作为一种利用肽辅助互补策略的无成熟报告器。使用从超过4000个候选文库对文库筛选中获得的有效二聚肽，实现了报告蛋白的快速激活。
Fluorescent protein-based reporter systems are used to track gene expression in cells. Here, we propose a modified bioluminescence resonance energy transfer (BRET) reporter as a maturation-less reporter that utilizes a peptide-assisted complementation strategy. Using effective dimerized peptides obtained from library-versus-library screening with more than 4000 candidates, rapid activation of the reporter was achieved.