Angiogenic activitiy of syndecan-binding laminin peptide AG73 (RKRLQVQLSIRT)
Angiogenic activitiy of syndecan-binding laminin peptide AG73 (RKRLQVQLSIRT)
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DOI：
10.1016/j.abb.2006.12.026
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发表时间：
2007-03-15
影响因子：
3.9
通讯作者：
Nomizu, Motoyoshi
中科院分区：
文献类型：
--
作者：
Mochizuki, Mayumi;Philp, Deborah;Nomizu, Motoyoshi
AG 73肽（RKRLQVQLSIRT，小鼠层粘连蛋白α 1链2719-2730）促进细胞粘附和肿瘤转移，并与跨膜多配体蛋白聚糖相互作用。在这里，我们使用体外、离体和体内模型证明了AG 73肽的血管生成活性。AG 73诱导小鼠内皮细胞（SVEC 4 -10）在Cultrex基底膜提取物（Cultrex BME）上形成管，并刺激主动脉环发芽。来自层粘连蛋白α 2、α 3、α 4或α 5链的同源序列在促进出芽形成方面都没有AG 73那么有活性。在鸡绒毛膜尿囊膜（CAM）测定中，AG 73还介导血管生成。使用皮下注射补充有AG 73的Cultrex BME，我们观察到大的血管生成反应。此外，AG 73-共轭壳聚糖膜促进CAM测定中的强血管生成反应。这些结果表明，AG 73肽是一种有效的多配体结合血管生成刺激剂，并可用于治疗缺血性损伤的治疗应用。(c)2007年爱思唯尔公司All rights reserved.
The AG73 peptide (RKRLQVQLSIRT, mouse laminin alpha 1 chain 2719-2730) promotes cell adhesion and tumor metastasis, and interacts with transmembrane syndecan proteoglycans. Here, we demonstrate AG73 peptide angiogenic activity using in vitro, ex vivo, and in vivo models. AG73 induced murine endothelial cell (SVEC4-10) tube formation on Cultrex Basement Membrane Extract (Cultrex BME) and stimulated sprouting of aortic rings. None of the homologous sequences from the laminin alpha 2, alpha 3, alpha 4, or alpha 5 chains was as active as AG73 in promoting sprouting formation. AG73 also mediated angiogenesis in the chick chorioallantonic membrane (CAM) assay. Using subcutaneously injected Cultrex BME supplemented with AG73, we observed a large angiogenic response. Furthermore, AG73-conjugated to a chitosan membrane promoted a strong angiogenic response in the CAM assay. These results indicate that the AG73 peptide is a potent syndecan-binding angiogenesis stimulator and may be useful for therapeutic application to treat ischemic injuries. (c) 2007 Elsevier Inc. All rights reserved.