MOLECULAR ANALYSIS AND DEVELOPMENTAL EXPRESSION OF THE FOCAL ADHESION KINASE PP125(FAK) IN XENOPUS-LAEVIS
MOLECULAR ANALYSIS AND DEVELOPMENTAL EXPRESSION OF THE FOCAL ADHESION KINASE PP125(FAK) IN XENOPUS-LAEVIS
复制标题
DOI：
10.1006/dbio.1995.1214
复制
发表时间：
1995-08-01
影响因子：
2.7
通讯作者：
DESIMONE, DW
中科院分区：
文献类型：
--
作者：
HENS, MD;DESIMONE, DW
整合素介导的细胞粘附到细胞外基质（ECM）的成分是重要的，在脊椎动物胚胎发生过程中发生的许多形态发生事件。最近的研究表明，粘着斑激酶pp 125（FAK）参与调节整合素依赖的信号传导过程触发细胞粘附到ECM。我们报道了爪蟾pp 125（FAK）同源基因的cDNA克隆和序列分析。我们还描述了FAK表达的时间和空间模式在早期发展。非洲爪蟾FAK与其鸟类和哺乳动物同源物具有超过90%的同一性。FAK mRNA和蛋白质存在于受精卵和卵裂期胚胎中。在原肠胚形成过程中，FAK蛋白表达显著增加，并在中胚层、边缘区外胚层和囊胚腔顶的细胞中检测到。在发育后期，FAK在体间连接处、脑中和几个颅神经中显著表达。磷酸化酪氨酰-FAK首先在原肠胚形成期间被检测到，这表明FAK在酪氨酸上的磷酸化是发育调节的。这些数据表明，FAK是可能参与各种整合素ECM依赖的信号事件在形态发生。(C)出版社：Academic Press
Integrin-mediated cellular adhesion to components of the extracellular matrix (ECM) is important in a number of morphogenetic events that occur during vertebrate embryogenesis. Recent studies suggest that the focal adhesion kinase pp125(FAK) is involved in the regulation of integrin-dependent signaling processes triggered by cell adhesion to the ECM. We report the cDNA cloning and sequence analysis of the Xenopus homolog of pp125(FAK). We also describe temporal and spatial patterns of FAK expression during early development. Xenopus FAK shares greater than 90% identity with its avian and mammalian homologs. FAK mRNA and protein are present in the fertilized egg and in cleavage stage embryos. During gastrulation, FAK protein expression increases significantly and is detected in mesoderm, marginal zone ectoderm, and cells of the blastocoel roof. Later in development, FAK is prominently expressed at intersomitic junctions, in the brain, and in several cranial nerves. Phosphotyrosyl-FAK is first detected during gastrulation, suggesting that the phosphorylation of FAK on tyrosine is developmentally regulated. These data indicate that FAK is likely to participate in a variety of integrin-ECM-dependent signaling events during morphogenesis. (C) 1995 Academic Press, Inc.