MONOCLONAL-ANTIBODIES IDENTIFY BLASTEMAL CELLS DERIVED FROM DEDIFFERENTIATING MUSCLE IN NEWT LIMB REGENERATION
MONOCLONAL-ANTIBODIES IDENTIFY BLASTEMAL CELLS DERIVED FROM DEDIFFERENTIATING MUSCLE IN NEWT LIMB REGENERATION
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DOI：
10.1038/308067a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
BROCKES, JP
中科院分区：
文献类型：
--
作者：
KINTNER, CR;BROCKES, JP
胚细胞是在有尾目两栖动物截肢或尾巴后产生的。这些组织学上未分化的间充质细胞分裂并随后分化以再生新的附属物。各种研究（参考文献 1 中的综述）表明，胚细胞起源于截肢部位附近的组织，包括肌肉、软骨、神经和真皮。然而，多核肌纤维是胚细胞的一个有争议的来源2。肌纤维可以去分化的建议是基于其在肢体再生早期阶段的组织学外观3-5。这与高等脊椎动物广泛接受的肌肉再生观点相反，后者将其归因于卫星细胞6,7。去分化假说的一个预测是，在再生的早期阶段应该存在具有肌纤维和芽基细胞特性的群体。在这里，我们描述了两种单克隆抗体的分离，一种识别仅在肌纤维中发现的抗原，另一种识别仅限于胚细胞的抗原。通过使用这些抗体作为细胞标记物，我们可以检测成年蝾螈再生肢体中携带肌纤维和胚细胞抗原的一小群细胞。这些双标记细胞的时间和位置支持了胚细胞部分起源于肌纤维去分化的观点。
Blastemal cells arise after the amputation of limbs or tails in urodele amphibians. These histologically undifferentiated mesenchymal cells divide and subsequently differentiate to regenerate a new appendage. Various studies (reviewed in ref. 1) indicate that blastemal cells arise from tissues near the site of amputation, including muscle, cartilage, nerve and dermis. The multinucleated myofibre, however, is a controversial source of blastemal cells2. The suggestion that myofibres can dedifferentiate is based on their histological appearance during the early stages of limb regeneration3–5. This is contrary to the widely accepted view of muscle regeneration in higher vertebrates which attributes it to satellite cells6,7. One prediction of the dedifferentiation hypothesis is that a population with properties of both myofibres and blastema cells should be present during the early stages of regeneration. Here we describe the isolation of two monoclonal antibodies, one that recognizes an antigen found only in myofibres and another that recognizes an antigen restricted to blastemal cells. By using these antibodies as cell markers, we can detect a small population of cells in the regenerating limbs of adult newts that bear both the myofibre and blastemal cell antigens. The time and location of these double-labelled cells supports the idea that blastemal cells originate, in part, by dedifferentiation of myofibres.