Proliferative lifespan is conserved after nuclear transfer
Proliferative lifespan is conserved after nuclear transfer
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DOI：
10.1038/ncb992
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发表时间：
2003-06-01
影响因子：
21.3
通讯作者：
Cui, W
中科院分区：
文献类型：
--
作者：
Clark, AJ;Ferrier, P;Cui, W
培养的原代细胞表现出有限的增殖寿命，称为Hayflick极限（1）。通过核移植进行克隆可以逆转这种细胞衰老过程，并且可以在接近衰老的培养细胞中完成（2）。在这里，我们描述了核移植实验中，供体细胞系在不同的年龄和不同的增殖能力被用来克隆胎儿和动物，从其中产生新的原代细胞系。再衍生的细胞系具有与供体细胞系相同的增殖能力和端粒缩短率，这表明这些是先天的、遗传决定的、通过核转移保守的特性。
Cultured primary cells exhibit a finite proliferative lifespan, termed the Hayflick limit(1). Cloning by nuclear transfer can reverse this cellular ageing process and can be accomplished with cultured cells nearing senescence(2). Here we describe nuclear transfer experiments in which donor cell lines at different ages and with different proliferative capacities were used to clone foetuses and animals from which new primary cell lines were generated. The rederived lines had the same proliferative capacity and rate of telomere shortening as the donor cell lines, suggesting that these are innate, genetically determined, properties that are conserved by nuclear transfer.