Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana.
Molecular characterization and immunohistochemical localization of a mitogen-activated protein kinase, Accp38b, from Apis cerana cerana.
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DOI：
10.5483/bmbrep.2012.45.5.293
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发表时间：
2012-05
期刊：
影响因子：
3.8
通讯作者：
Liang Zhang;F. Meng;Yuzhen Li;M. Kang;Xingqi Guo;Baohua Xu
中科院分区：
文献类型：
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作者：
Liang Zhang;F. Meng;Yuzhen Li;M. Kang;Xingqi Guo;Baohua Xu
p38丝裂原活化蛋白激酶（MAPK）参与多种过程，包括应激反应、发育和分化。然而，关于昆虫中p38 MAPK的信息很少。本研究从中华蜜蜂中克隆了一个p38 MAPK基因Accp 38 b，并对其进行了鉴定。实时荧光定量PCR（Q-PCR）分析表明，Accp 38 b受多种应激诱导表达。值得注意的是，Accp 38 b的表达在蛹期比在其他发育阶段相对较高。在蛹期，Accp 38 b在胸部的表达高于头和腹部，在脂肪体中的表达高于肌肉和中肠。免疫组化结果显示，Accp 38 b在A.中华蜜蜂。这些结果表明Accp 38 b可能在应激反应中起重要作用，并在发育过程中具有多方面的功能。
The p38 mitogen-activated protein kinase (MAPK) is involved in various processes, including stress responses, development, and differentiation. However, little information on p38 MAPK in insects is available. In this study, a p38 MAPK gene, Accp38b, was isolated from Apis cerana cerana and characterized. The quantitative real-time PCR (Q-PCR) analysis revealed that Accp38b was induced by multiple stressors. Notably, the expression of Accp38b was relatively higher in the pupae phase than in other developmental phases. During the pupae phase, Accp38b expression was higher in the thorax than in the head and abdomen and higher in the fat body than in the muscle and midgut. Immunohistochemisty showed significant positive staining of Accp38b in sections from the brain, eyes, fat body, and midgut of A. cerana cerana. These results suggest that Accp38b may play a crucial role in stress responses and have multiple aspects function during development.