The AP2/ERF transcription factor SlERF.F5 functions in leaf senescence in tomato
The AP2/ERF transcription factor SlERF.F5 functions in leaf senescence in tomato
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DOI：
10.1007/s00299-022-02846-1
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发表时间：
2022-03-03
影响因子：
6.2
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
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作者：
Chen, Yanan;Feng, Panpan;Chen, Guoping
我们的研究结果证实了SlERF. F5可以直接调节ACS 6的启动子活性，并与SlMYC 2相互作用来调节番茄叶片衰老。植物衰老过程是一个复杂的、高度协调的过程，受多种内源和环境信号的调控。乙烯和茉莉酸是众所周知的衰老诱导剂，但它们诱导叶片衰老的分子机制尚未完全阐明。在这里，我们从番茄中分离出了乙烯反应因子F5（SLERF.F5）。SLERF. F5的沉默导致由年龄、黑暗、乙烯和茉莉酸诱导的加速衰老。然而，SlERF.F5的过表达不会促进衰老。此外，SlERF.F5可以在体外和体内调节ACS 6的启动子活性。抑制SLERF. F5导致对乙烯和茉莉酸的敏感性增加，叶绿素含量的积累减少，并抑制叶绿素和光响应相关基因的表达。qRT-PCR分析表明，与野生型相比，SLERF. F5-RNAi株系中乙烯生物合成途径和茉莉酸信号途径相关基因的表达水平均有所提高。酵母双杂交实验表明，SlERF. F5和SlMYC 2（JA受体下游的转录因子）可以物理相互作用，从而介导SlERF. F5在茉莉酸诱导的叶片衰老中的作用。总的来说，我们的研究为乙烯和茉莉酸如何促进番茄叶片衰老提供了新的见解。
Key message Our results confirmed that SlERF.F5 can directly regulate the promoter activity of ACS6 and interact with SlMYC2 to regulate tomato leaf senescence. The process of plant senescence is complex and highly coordinated, and is regulated by many endogenous and environmental signals. Ethylene and jasmonic acid are well-known senescence inducers, but their molecular mechanisms for inducing leaf senescence have not been fully elucidated. Here, we isolated an ETHYLENE RESPONSE FACTOR F5 (SlERF.F5) from tomato. Silencing of SlERF.F5 causes accelerated senescence induced by age, darkness, ethylene, and jasmonic acid. However, overexpression of SlERF.F5 would not promote senescence. Moreover, SlERF.F5 can regulate the promoter activity of ACS6 in vitro and in vivo. Suppression of SlERF.F5 resulted in increased sensitivity to ethylene and jasmonic acid, decreased accumulation of chlorophyll content, and inhibited the expression of chlorophyll- and light response-related genes. Compared with the wild type, the qRT-PCR analysis showed the expression levels of genes related to the ethylene biosynthesis pathway and the jasmonic acid signaling pathway in SlERF.F5-RNAi lines increased. Yeast two-hybrid experiments showed that SlERF.F5 and SlMYC2 (a transcription factor downstream of the JA receptor) can interact physically, thereby mediating the role of SlERF.F5 in jasmonic acid-induced leaf senescence. Collectively, our research provides new insights into how ethylene and jasmonic acid promote leaf senescence in tomato.