Estradiol regulates the transcription of the prolactin gene.
Estradiol regulates the transcription of the prolactin gene.
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DOI：
10.1016/s0021-9258(18)34893-2
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发表时间：
1982-03
期刊：
影响因子：
--
通讯作者：
R. Maurer
中科院分区：
文献类型：
--
作者：
R. Maurer
研究了雌二醇对去卵巢雌性大鼠垂体泌乳素基因转录的影响。对垂体细胞中存在的多种催乳素mRNA的分析表明，雌二醇增加了所有大的、潜在的催乳素mRNA的核前体的浓度。为了进一步研究雌二醇对催乳素mRNA合成的影响，从大鼠垂体中分离细胞核，并在[α - 32p]UTP存在的情况下继续体外RNA合成。将[32P]RNA与含有催乳素cDNA序列的固定质粒DNA杂交，定量分析新合成的催乳素mRNA序列。雌二醇处理24 h后，催乳素mRNA合成增加4倍。雌二醇对催乳素基因转录的影响早在注射类固醇后20分钟检测到。这些发现表明雌二醇通过增加催乳素基因的转录来增加催乳素mRNA水平。
The effect of estradiol on prolactin gene transcription in pituitaries from ovariectomized, female rats has been investigated. Analysis of the multiple prolactin mRNA species present in pituitary cells demonstrated that estradiol increases the concentration of all the large, potential nuclear precursors of prolactin mRNA. To further examine possible estradiol effects on prolactin mRNA synthesis, nuclei were isolated from rat pituitaries and allowed to continue RNA synthesis in vitro in the presence of [alpha-32P]UTP. Newly synthesized prolactin mRNA sequences were quantitated by hybridization of [32P]RNA to immobilized plasmid DNA containing prolactin cDNA sequences. After 24 h of estradiol treatment, prolactin mRNA synthesis was increased 4-fold. Estradiol effects on prolactin gene transcription were detected as early as 20 min after injection of the steroid. These findings suggest that estradiol increases prolactin mRNA levels by increasing the transcription of the prolactin gene.