Simultaneous determination of vancomycin and ceftazidime in cerebrospinal fluid in craniotomy patients by high-performance liquid chromatography
Simultaneous determination of vancomycin and ceftazidime in cerebrospinal fluid in craniotomy patients by high-performance liquid chromatography
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DOI：
10.1016/j.jpba.2008.06.012
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发表时间：
2008-11-04
影响因子：
3.4
通讯作者：
Jiang, Yunyun
中科院分区：
文献类型：
--
作者：
Ye, Guangming;Cai, Xuejian;Jiang, Yunyun
一种利用高效液相色谱 (HPLC) 同步分析脑脊液 (CSF) 中万古霉素和头孢他啶的简单、准确和快速的方法已经开发出来并经过彻底验证，以满足严格的 FDA 生物分析方法指南。采用蛋白质沉淀作为样品前处理方法。为了提高准确性，选择替硝唑作为内标。使用由乙腈和醋酸盐缓冲液(pH 3.5)(8:92，v/v)组成的流动相，在室温25℃下，在Diamonsil C18柱(200mm x 4.6mm内径，5μm)上实现分离，检测波长为240nm。所有验证数据，例如准确度、精度、钻孔日间重复性，均在要求的范围内。该方法用于测定五名开颅手术患者脑脊液中万古霉素和头孢他啶的浓度。 (C) 2008 Elsevier B.V. 保留所有权利。
A simple, accurate and rapid method for simultaneous analysis of vancomycin and ceftazidime in cerebrospinal fluid (CSF), utilizing high-performance liquid chromatography (HPLC), has been developed and thoroughly validated to satisfy strict FDA guidelines for bioanalytical methods. Protein precipitation was used as the sample pretreatment method. In order to increase the accuracy, tinidazole was chosen as the internal standard. Separation was achieved on a Diamonsil C18 column (200 mm x 4.6 mm I.D., 5 mu m) using a mobile phase composed of acetonitrile and acetate buffer (pH 3.5) (8:92, v/v) at room temperature 25 degrees C and the detection wavelength was 240 nm. All the validation data, such as accuracy, precision, drill inter-day repeatability, were within the required limits. The method was applied to determine vancomycin and ceftazidime concentrations in CSF in five craniotomy patients. (C) 2008 Elsevier B.V. All rights reserved.