Effects of dexamethasone and protein kinase C inhibitors on the induction of bradykinin B1 mRNA and the bradykinin B1 receptor-mediated contractile response in isolated rat ileum.
Effects of dexamethasone and protein kinase C inhibitors on the induction of bradykinin B1 mRNA and the bradykinin B1 receptor-mediated contractile response in isolated rat ileum.
复制标题
地塞米松和蛋白激酶 C 抑制剂对离体大鼠回肠中缓激肽 B1 mRNA 诱导和缓激肽 B1 受体介导的收缩反应的影响。
DOI：
10.1016/s0006-2952(02)00905-x
复制
发表时间：
2002
影响因子：
5.8
通讯作者：
S. Oh‐ishi
中科院分区：
文献类型：
--
作者：
A. Ueno;E. Dekura;Y. Kosugi;M. Yoshimura;H. Naraba;Fumiaki Kojima;S. Oh‐ishi
采用逆转录-聚合酶链式反应(RT-PCR)方法，检测37°充气的泰氏液中悬吊至少1小时大鼠回肠组织中可诱导的缓激肽(BK)B1受体mRNA的表达。DES-Arg9-BK对该基因的诱导具有时间和温度依赖性，在孵育约3小时后出现收缩反应；这种反应随着时间的延长而增加，并在6小时时达到最大。相反，对BK的收缩反应和B2受体mRNA的表达在这6小时的潜伏期内是恒定的。由DES-Arg9-BK引起的收缩可被B1受体拮抗剂DES-Arg9[Leu8]-BK和Des-Arg10-HOE140选择性地抑制，但不能被B2受体拮抗剂d-Arg-[Hyp3，Thi5，8，d-Phe7]-BK和HOE140所抑制。回肠持续暴露于放线菌素D或放线菌素D可抑制DES-Arg9-BK可诱导的收缩反应，但这两种抑制剂均不影响BK的收缩反应，提示B1受体可被从头开始诱导。地塞米松在体外和体外处理回肠均可抑制DES-Arg9-BK引起的收缩反应，但对B1受体基因表达无明显影响。一些蛋白激酶C抑制剂，如H7和Calphostin C，可抑制B1受体mRNA的表达，并减弱对DES-Arg9-BK的收缩反应。这些结果表明，回肠B1受体的从头合成可以在转录水平上上调(这一过程可能涉及一种特定的蛋白激酶C亚型)。此外，这些数据表明，对DES-Arg9-BK的收缩反应涉及对地塞米松转录后作用敏感的过程。
We detected the expression of inducible bradykinin (BK) B1receptor mRNA in the rat ileum by the reverse transcriptase–polymerase chain reaction (RT–PCR) method, when the isolated ileum was suspended for at least 1hr in an aerated Tyrode’s solution at 37°. The induction of this mRNA was both time- and temperature-dependent, and was followed by a contractile response to des-Arg9-BK at around 3hr of incubation; this response increased in magnitude with time and was maximal at 6hr. In contrast, the contraction in response to BK and the expression of B2receptor mRNA were constant throughout this 6-hr incubation period. The contraction due to des-Arg9-BK was selectively suppressed by B1receptor antagonists, i.e. des-Arg9[Leu8]-BK and des-Arg10-HOE140, but not by the B2antagonists d-Arg-[Hyp3,Thi5,8,d-Phe7]-BK and HOE140. The inducible des-Arg9-BK contractile response was suppressed by continuous in vitro exposure of the ileum to cycloheximide or actinomycin D, but neither inhibitor affected the contraction induced by BK, suggesting that the B1receptor could be induced de novo. In vitro and ex vivo treatment of the ileum with dexamethasone suppressed the induction of the contractile response to des-Arg9-BK, but had no significant effect on the expression of B1receptor mRNA. Some protein kinase C inhibitors, i.e. H7 and calphostin C, suppressed the expression of B1receptor mRNA and diminished the contractile response to des-Arg9-BK. These results suggest that the de novo synthesis of the B1receptor in the ileum preparation can be up-regulated at the transcriptional level (a process in which a specific isoform of protein kinase C may be involved). Additionally, these data suggest that the contractile response to des-Arg9-BK involves a process sensitive to some post-transcriptional action of dexamethasone.
登录
查看更多内容
DOI：
10.1073/pnas.88.17.7724
发表时间：
1991-09-01
影响因子：
11.1
作者：
MCEACHERN, AE;SHELTON, ER;JARNAGIN, K
通讯作者：
JARNAGIN, K
通过 des-Arg9-缓激肽刺激 B1 受体来舒张孤立的肠系膜动脉。
影响因子：
5
作者：
Churchill,L;Ward,PE
通讯作者：
Ward,PE
大鼠缓激肽B1受体的分子克隆和表达。
DOI：
10.1016/s0167-4781(98)00163-8
发表时间：
1998
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Ni,A;Chai,KX;Chao,L;Chao,J
通讯作者：
Chao,J
IL-1β、佛波醇酯和转录后调节因子在控制缓激肽 B1 受体基因表达中的作用。
DOI：
10.1042/bj3300361
发表时间：
1998
期刊：
The Biochemical journal
影响因子：
--
作者：
Zhou,X;Polgar,P;Taylor,L
通讯作者：
Taylor,L