CK7+/CK20- immunoexpression profile is typical of salivary gland neoplasia
CK7+/CK20- immunoexpression profile is typical of salivary gland neoplasia
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DOI：
10.1111/j.1365-2559.2007.02728.x
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发表时间：
2007-07-01
期刊：
影响因子：
6.4
通讯作者：
Altini, M.
中科院分区：
文献类型：
--
作者：
Meer, S.;Altini, M.
目的：评估细胞角蛋白（CK）7/20表达模式在涎腺neoplasia.Methods和结果：福尔马林固定石蜡包埋组织从153涎腺肿瘤进行了CK 7/20免疫反应性评价。其中多形性腺瘤24例，肌上皮瘤9例，乳头状囊腺瘤3例，嗜酸细胞瘤2例，腺样囊性癌22例，粘液表皮样癌21例，多形性低度恶性腺癌21例，癌外多形性腺瘤11例，腺泡细胞癌17例，表膜上皮癌（n = 7）、嗜酸细胞癌（n = 3）、透明化透明细胞癌（n = 1）、乳头状囊腺癌（n = 1）、涎腺导管癌（n = 3）、腺癌（未另外说明）（n = 4）和鳞状细胞癌（n = 4）。在适当的对照存在下，使用单克隆抗体CK 7（OV-TL 12/30）、CK 20（Ks 20.8）和M3515细胞角蛋白（AE 1/AE 3）进行免疫组织化学程序。根据阳性肿瘤细胞的估计百分比，半定量地表示结果：1+，5-25%; 2+，26-75%;和3+，76- 100%。所有唾液腺肿瘤均显示CK 7 +/CK 20-免疫特征，范围为5 - 100%。结论：CK 7/20免疫组化在涎腺肿瘤的良恶性鉴别诊断中无明显意义，但对原发性涎腺肿瘤与转移性肿瘤、鳞癌的鉴别诊断及转移性涎腺肿瘤的诊断有一定的参考价值。
Aims: To evaluate cytokeratin (CK) 7/20 expression patterns in salivary gland neoplasia.Methods and results: Formalin-fixed paraffin embedded tissue from 153 salivary gland tumours were evaluated for CK7/20 immunoreactivity. The tumours included pleomorphic adenoma (n = 24), myoepithelioma (n = 9), papillary cystadenoma (n = 3), oncocytoma (n = 2), adenoid cystic carcinoma (n = 22), mucoepidermoid carcinoma (n = 21), polymorphous low-grade adenocarcinoma (n = 21), carcinoma ex-pleomorphic adenoma (n = 11), acinic cell carcinoma (n = 17), epimyoepithelial carcinoma (n = 7), oncocytic carcinoma (n = 3), hyalinizing clear cell carcinoma (n = 1), papillary cystadenocarcinoma (n = 1), salivary duct carcinoma (n = 3), adenocarcinoma ( not otherwise specified) (n = 4) and squamous carcinoma (n = 4). Immunohistochemical procedures were performed using monoclonal antibodies CK7 (OV-TL 12/30), CK20 (Ks 20.8) and M3515 cytokeratin (AE1/AE3) in the presence of appropriate controls. The results were expressed semiquantitatively, according to the estimated percentage of positive tumour cells: 1+, 5-25%; 2+, 26-75%; and 3+, 76-100%. All salivary gland neoplasms showed a CK7+/CK20- immunoprofile ranging from 5 to 100%. Squamous carcinoma showed negative CK7/20 immunoexpression.Conclusions: Although the CK7/20 immunoprofile is not useful in distinguishing the various types of salivary gland neoplasms or between benign and malignant salivary gland tumours, it may facilitate differentiation of primary salivary gland neoplasia from metastatic tumours and squamous carcinoma, and the diagnosis of metastatic salivary gland tumours.