ISOLATION OF 6 NOVEL PHOSPHOINOSITOL-CONTAINING SPHINGOLIPIDS FROM TOBACCO-LEAVES
ISOLATION OF 6 NOVEL PHOSPHOINOSITOL-CONTAINING SPHINGOLIPIDS FROM TOBACCO-LEAVES
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DOI：
10.1021/bi00610a023
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
LESTER, RL
中科院分区：
文献类型：
--
作者：
KAUL, K;LESTER, RL
用二乙胺乙基纤维素层析法将烟草叶片磷脂浓缩物分为两组。洗脱的第一组不含乙酰基残基，而第二组含有1个n -乙酰基/mol P.每组3个脂质通过Porasil柱层析纯化。这6种新型脂质的化学成分表明它们与先前表征的磷脂类磷脂psl - 1， n -乙酰氨基葡萄糖[葡萄糖醛酸，肌醇磷酸]神经酰胺和PSL-II，氨基葡萄糖[葡萄糖醛酸，肌醇磷酸]神经酰胺有关。新型脂质有：PSL-IA、PSL-I-[Ara2Gal2]；PSL-IB PSL-I——[Ara3Gal2];PSL-IC PSL-I——[Ara4Gal2];PSL-IIA PSL-II——[Ara3Gal];PSL-IIB PSL-II——[Ara2or3Gal2];PSL IIC, PSL- ii -[Ara2Gal2Man]。在PSL-II组脂质中不寻常的发现或未乙酰化的己糖胺似乎不是人为的，正如涉及烟叶快速收获和加工的实验所表明的那样。在二乙胺乙基纤维素层析上得到的一些组分似乎含有含有16个或更多糖残基的磷脂。
A concentrate of phosphosphingolipids from tobacco [Nicotiana tabacum] leaves was resolved into 2 groups by chromatography on diethylaminoethylcellulose. The first group eluted contains no acetyl residues, whereas the second group contains one N-acetyl/mol of P. Three lipids from each group were purified by chromatography on Porasil columns. The chemical composition of these 6 novel lipids shows them to be related to the previously characterized phosphosphingolipids PSL-I, N-acetylglucosamine[glucuronic acid, inositol phosphate]ceramide and PSL-II, glucosamine[glucuronic acid, inositol phosphate]ceramide. The novel lipids are: PSL-IA, PSL-I-[Ara2Gal2]; PSL-IB, PSL-I-[Ara3Gal2]; PSL-IC, PSL-I-[Ara4Gal2]; PSL-IIA, PSL-II-[Ara3Gal]; PSL-IIB, PSL-II-[Ara2or3Gal2]; PSL IIC, PSL-II-[Ara2Gal2Man]. The unusual finding or unacetylated hexosamine in the PSL-II group of lipids appears not to be an artifact, as suggested by experiments involving rapid harvesting and processing of the tobacco leaves. Some of the fractions obtained by chromatography on diethylaminoethylcellulose appear to contain phosphosphingolipids with 16 or more sugar residues.