[Immune response against BCG 65 kDa protein antigen in bladder cancer with BCG instillation therapy. Analyses carried out with the gene of this protein to develop a monoclonal antibody].
[Immune response against BCG 65 kDa protein antigen in bladder cancer with BCG instillation therapy. Analyses carried out with the gene of this protein to develop a monoclonal antibody].
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[卡介苗滴注疗法对膀胱癌中卡介苗 65 kDa 蛋白抗原的免疫反应。
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Kamidono
中科院分区：
文献类型：
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作者：
I. Hara;Noriyuki Sato;K. Kikuchi;S. Kamidono
BCG 65 kDa蛋白是60 kDa热休克蛋白（hsp）家族的一部分。很明显，热休克蛋白在哺乳动物和细菌细胞中具有巨大的同源性。在我们的研究中，我们观察到这种蛋白质在抗肿瘤反应中的相关性。使用51名接受BCG滴注治疗的膀胱癌患者的血清，通过固相ELISA测定针对BCG 65 kDa蛋白的抗体产生。BCG 65 kDa蛋白由E.用编码该蛋白的pTB 12（质粒DNA）转染的大肠杆菌。可以清楚地观察到，通过上述滴注治疗，针对该蛋白质的血清抗体滴度水平升高。为了研究该蛋白在抗肿瘤反应中的作用，我们开发了针对BCG 65 kDa蛋白的单克隆抗体。开发了四种单克隆抗体（B-20、B-97、B-108、B-167）。我们还利用大肠杆菌产生的截短蛋白估算了每种单克隆抗体的表位。用pTB 12缺失突变体转染大肠杆菌。在这个阶段，我们继续观察使用这些单克隆抗体后哺乳动物癌细胞和BCG之间的交叉表位。哺乳动物细胞中不存在由B-20、B-97和B-167定义的交叉表位。然而，由B-108定义的表位存在于正常组织以及膀胱癌细胞中。
The BCG 65 kDa protein is part of the 60 kDa heat-shock protein (hsp) family. It is clear that hsp has a huge homology among mammalian and bacterial cells. In our study, we have observed the relevance of this protein in the anti-tumor response. Antibody production against the BCG 65 kDa protein was determined by solid phase ELISA using the sera of 51 bladder cancer patients who have undergone BCG instillation therapy. The BCG 65 kDa protein was made by E. coli transfected with pTB12 (plasmid DNA) which encodes this protein. It was clearly observed that the level of serum antibody titer to this protein was raised by the above instillation therapy. In order to investigate the role of this protein in anti-tumor response, we developed monoclonal antibodies against BCG 65 kDa protein. Four monoclonal antibodies were developed (B-20, B-97, B-108, B-167). We also estimated the epitope defined by each monoclonal antibody by using the truncated protein which was produced by E. coli transfected with the deletion mutants of pTB12. At this stage, we proceeded with observation of the cross epitope between mammalian cancer cells and BCG after using these monoclonal antibodies. There is no cross epitope defined by B-20, B-97 and B-167 in mammalian cells. However, the epitope defined by B-108 exists in normal tissue as well as in bladder cancer cells.
应激蛋白是麻风病和结核病的免疫靶标。
DOI：
10.1073/pnas.85.12.4267
发表时间：
1988
影响因子：
11.1
作者：
Young,D;Lathigra,R;Hendrix,R;Sweetser,D;Young,RA
通讯作者：
Young,RA
膀胱癌患者免疫治疗期间对卡介苗的抗体反应。
影响因子：
11.2
作者：
Winters,WD;Lamm,DL
通讯作者：
Lamm,DL
应激蛋白、关节炎和自身免疫。
影响因子：
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作者：
Winfield,JB
通讯作者：
Winfield,JB