Observations on experimental marginal periodontitis in rats
Observations on experimental marginal periodontitis in rats
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DOI：
10.1111/j.1600-0765.2004.00710.x
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发表时间：
2004-04-01
影响因子：
3.5
通讯作者：
Kocher, T
中科院分区：
文献类型：
--
作者：
Kuhr, A;Popa-Wagner, A;Kocher, T
目的：通过60天的观察观察结扎法对实验性周缘性牙周炎大鼠牙周损害的影响。在何种程度上，生理运动的牙齿影响结扎的效果也进行了检查。此外，比较了两种测量脱牙颌骨损失的方法。方法：35只雄性SD大鼠分为5组。上颌第二磨牙结扎引起边缘牙周炎。15、30和60天后处死老鼠。对照组大鼠分别于第1天和第60天处死。用两种不同的方法测定脱落颌骨的颊面和腭面骨质流失。在第一种方法中，在不同位置测量牙髓牙釉质连接（CEJ）到牙槽骨嵴（ABC）的距离；第二种方法是测量磨牙暴露的根面面积。结果：两种测量方法在第1天和第60天的对照组比较，骨质流失有显著差异。在结扎处，实验组大鼠的骨质流失明显大于对照组大鼠。第1天的对照大鼠与第15天的试验大鼠比较，结扎区域内各组间骨质流失的增加明显大于结扎区域外。在整个60天的观察期内，年龄依赖性骨质流失增加。结扎引起的骨质流失在第1天至第15天增加最多；在第30和60天，观察到骨质流失略有增加。结论：该模型仅适用于较短（15天）的观察期。距离法应优于面积法。
Objective: The purpose of this study was to assess periodontal destruction following experimentally induced marginal periodontitis in rats by ligatures over a 60-day observation period. The extent to which the physiological movement of teeth influenced the effect of the ligatures was also examined. In addition, two methods for measuring bone loss in the defleshed jaw were compared.Methods: Thirty-five male Sprague-Dawley rats (SD) were divided into five groups. Marginal periodontitis was induced by ligatures on the second maxillary molars. Rats were killed after 15, 30, and 60 days. Rats in the control group were killed on day 1 and day 60. Bone loss was determined with two different methods on the buccal and palatinal surfaces of the defleshed jaw. In the first method, the distance of the cementoenamel junction (CEJ) from the alveolar bone crest (ABC) was measured at different sites; in the second method, the area of the exposed root surface of the molars was measured.Results: Comparison of the control groups from day 1 and day 60 using both measuring methods showed significant differences in bone loss. In the area where the ligature was located, test rats exhibited significantly greater bone loss than control rats. Comparison of control rats from day I with test rats from day 15 showed that the increase in bone loss between the groups within the area of the ligature was significantly greater than outside it. The age-dependent bone loss increases over the entire observation period of 60 days. The ligature-induced bone loss increased most from day 1 to day 15; on days 30 and 60, slighter increases in bone loss were observed.Conclusions: The application of this model can only be recommended for short ( 15 days) observation periods. The distance method should be preferred to the area method.