Permanent, nonleaching antibacterial surfaces. 1. Synthesis by atom transfer radical polymerization
Permanent, nonleaching antibacterial surfaces. 1. Synthesis by atom transfer radical polymerization
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DOI：
10.1021/bm034352k
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发表时间：
2004-05-01
影响因子：
6.2
通讯作者：
Russell, AJ
中科院分区：
文献类型：
--
作者：
Lee, SB;Koepsel, RR;Russell, AJ
我们使用原子转移自由基聚合（ATRP）直接在玻璃和纸张表面上生长抗菌聚合物。这里描述的方法导致潜在的永久性非浸出抗菌表面，而不需要化学接枝抗菌材料的基层。通过原子转移自由基聚合将甲基丙烯酸叔气2-（二甲基氨基）乙酯直接聚合到Whatman#1滤纸或载玻片上。在聚合之后，使用烷基卤化物将叔氨基季铵化以在聚合物改性的表面上产生大浓度的季铵基团。将改性材料与大肠杆菌或枯草杆菌一起孵育表明，改性表面具有显著的抗微生物能力。通过重复使用改性玻璃而没有显著的活性损失，证明了抗微生物活性的持久性。季胺被认为通过破坏细胞膜允许细胞内内容物的释放而引起细胞死亡。细胞在改性玻璃表面上的原子力显微镜成像支持这一假设。
We have grown an antimicrobial polymer directly on the surfaces of glass and paper using atom transfer radical polymerization (ATRP). The method described here results in potentially Permanent nonleaching antibacterial Surfaces Without the need to chemically graft the antimicrobial material to the substratum. The tertiary airline 2-(dimethylamino)ethyl methacrylate was polymerized directly onto Whatman #1 filter paper or glass slides via atom transfer radical polymerization. Following the polymerization, the tertiary amino groups were quaternized using an alkyl halide to produce a large concentration of quaternary ammonium groups on the polymer-modified surfaces. Incubating the modified materials with either Escherichia coli or BaCiffiff subtilis demonstrated that the modified surfaces had substantial antimicrobial capacity. The permanence of the antimicrobial activity was demonstrated through repeated use of a modified glass without significant loss of activity. Quaternary amines are believed to cause cell death by disrupting cell membranes allowing release of the intracellular contents. Atomic force microscopic imaging of cells on modified glass surfaces Supports this hypothesis.