Insulin-Sensitizing Effects on Muscle and Adipose Tissue after Dietary Fiber Intake in Men and Women with Metabolic Syndrome
Insulin-Sensitizing Effects on Muscle and Adipose Tissue after Dietary Fiber Intake in Men and Women with Metabolic Syndrome
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DOI：
10.1210/jc.2012-1513
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发表时间：
2012-09-01
影响因子：
5.8
通讯作者：
Umpleby, A. Margot
中科院分区：
文献类型：
--
作者：
Robertson, M. Denise;Wright, John W.;Umpleby, A. Margot
内容：膳食纤维与2型糖尿病发病率降低的流行病学研究，然而，确切的机制是unknown.目的：本研究的目的是评估来自玉米的不溶性膳食纤维（HAM-RS 2）在改善2型糖尿病风险增加的受试者的胰岛素抵抗方面的功效和作用部位。设计：本研究是一项随机、对照交叉、饮食干预研究。设置：本研究在英国吉尔福德的皇家萨里县医院糖尿病、内分泌学和研究中心进行。参与者：15名胰岛素抵抗的男性和女性参与了本研究。干预：干预包括40 g/d HAM-RS 2与匹配的安慰剂相比，持续8 wk.Main Outcome Measures：每次补充后，参与者进行了两步高胰岛素-正葡萄糖钳夹研究，并加入葡萄糖示踪剂;膳食耐量试验;前臂肌肉组织动静脉取样;结果：通过动静脉取样测量，前臂肌肉对葡萄糖的摄取增强（抗性淀粉后增加65 +/-15%; P < 0.001）。脂肪组织功能也受到影响，HAM-RS 2治疗后脂肪酸抑制增强，活检样本中激素敏感脂肪酶（P = 0.005）、围脂蛋白（P = 0.011）、脂蛋白脂肪酶（P = 0.014）和脂肪甘油三酯脂肪酶（P = 0.03）的基因表达增加。结论：HAM-RS 2可改善外周胰岛素抵抗，但不能改善肝脏胰岛素抵抗，作为2型糖尿病患者或有2型糖尿病风险患者的干预措施，尚需进一步研究。（临床内分泌代谢杂志97：3326-3332，2012）
Context: Dietary fibers have been associated with a reduced incidence of type 2 diabetes mellitus in epidemiological studies; however, the precise mechanisms are unknown.Objective: The objective of the study was to evaluate the efficacy and site of action of an insoluble dietary fiber derived from maize (HAM-RS2) in improving insulin resistance in subjects at increased risk of type 2 diabetes mellitus.Design: This study was a randomized, controlled crossover, dietary intervention study.Setting: The study was conducted at the Centre for Diabetes, Endocrinology, and Research, Royal Surrey County Hospital, Guildford, United Kingdom.Participants: Fifteen men and women with insulin resistance participated in the study.Intervention: The intervention included 40 g/d HAM-RS2 compared with a matched placebo for 8 wk.Main Outcome Measures: After each supplement, participants underwent a two-step hyperinsulinemic-euglycemic clamp study with the addition of glucose tracers; a meal tolerance test; arteriovenous sampling across forearm muscle tissue; and a sc adipose tissue biopsy for assessment of gene expression.Results: There was enhanced uptake of glucose into the forearm muscle measured by arteriovenous sampling (65 +/- 15% increase after resistant starch; P < 0.001). Adipose tissue function was also affected, with enhanced fatty acid suppression after HAM-RS2 treatment and an increase in gene expression for hormone sensitive lipase (P = 0.005), perilipin (P = 0.011), lipoprotein lipase (P = 0.014), and adipose triglyceride lipase (P = 0.03) in biopsy samples. There was no effect on the insulin sensitivity of hepatic glucose production or plasma lipids after HAM-RS2.Conclusion: HAM-RS2 improved peripheral but not hepatic insulin resistance and requires further study as an intervention in patients with or at risk for type 2 diabetes. (J Clin Endocrinol Metab 97: 3326-3332, 2012)