Parallel inhibition of active force and relaxed fiber stiffness in skeletal muscle by caldesmon: implications for the pathway to force generation.
Parallel inhibition of active force and relaxed fiber stiffness in skeletal muscle by caldesmon: implications for the pathway to force generation.
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卡德斯蒙对骨骼肌主动力和松弛纤维刚度的并行抑制：对力产生途径的影响。
DOI：
10.1073/pnas.88.13.5739
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发表时间：
1991
影响因子：
11.1
通讯作者：
Chalovich,JM
中科院分区：
文献类型：
--
作者：
Brenner,B;Yu,LC;Chalovich,JM
在最近关于肌肉收缩的假说中，肌球蛋白在两种类型的肌动蛋白结合构型之间循环。这两种构型在其肌动蛋白-肌球蛋白复合体(“弱结合”与“强结合”)的稳定性方面有很大差异，力的产生或运动是与从弱结合(力产生)构型向强结合(力产生)构型转变相关的结构变化的结果。Eisenberg，E.&Hill，T.L.(1985)科学227,999-1006]。具体地说，在这个概念中，主要的力产生状态只有在弱结合构型的初始跨桥连接之后才能到达。已经表明，在肌肉纤维中可以发生强的和弱的交叉桥附着[Brenner，B.，Schoenberg，M.，Chalovich，J.M.，Greene，L.E.&Eisenberg，E.(1982)Proc.娜塔莉。阿卡德。SCI。美国79,7288-7291]。然而，没有证据表明在弱结合状态下的依附是导致力产生的关键步骤。研究表明，钙调蛋白可用于选择性地抑制骨骼肌中弱结合交叉桥的附着。这种抑制会导致主动力的平行下降，而跨桥翻转的动力学不会因此而改变。这表明(I)弱结合状态下的跨桥连接是特定的，(Ii)只有在跨桥以弱结合、非力产生的构型与肌动蛋白连接之后，才能产生力。
In recent hypotheses on muscle contraction, myosin cross-bridges cycle between two types of actin-bound configuration. These two configurations differ greatly in the stability of their actin-myosin complexes ("weak-binding" vs. "strong-binding"), and force generation or movement is the result of structural changes associated with the transition from the weak-binding (preforce generating) configuration to strong-binding (force producing) configuration [cf. Eisenberg, E. & Hill, T. L. (1985) Science 227, 999-1006]. Specifically, in this concept, the main force-generating states are only accessible after initial cross-bridge attachment in a weak-binding configuration. It has been shown that strong and weak cross-bridge attachment can occur in muscle fibers [Brenner, B., Schoenberg, M., Chalovich, J. M., Greene, L. E. & Eisenberg, E. (1982) Proc. Natl. Acad. Sci. USA 79, 7288-7291]. However, there has been no evidence that attachment in the weak-binding states represents an essential step leading to force generation. It is shown here that caldesmon can be used to selectively inhibit attachment of weak-binding cross-bridges in skeletal muscle. Such inhibition causes a parallel decrease in active force, while the kinetics of cross-bridge turnover are unchanged by this procedure. This suggests that (i) cross-bridge attachment in the weak-binding states is specific and (ii) force production can only occur after cross-bridges have first attached to actin in a weakly bound, nonforce-generating configuration.
DOI：
10.1038/newbio238097a0
发表时间：
1972-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
BREMEL, RD;WEBER, A
通讯作者：
WEBER, A
DOI：
10.1101/sqb.1973.037.01.046
发表时间：
1973-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
HUXLEY, HE
通讯作者：
HUXLEY, HE
DOI：
10.1101/sqb.1973.037.01.044
发表时间：
1973-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
HASELGROVE, JC
通讯作者：
HASELGROVE, JC