Inducible Cx40-Cre Expression in the Cardiac Conduction System and Arterial Endothelial Cells
Inducible Cx40-Cre Expression in the Cardiac Conduction System and Arterial Endothelial Cells
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DOI：
10.1002/dvg.20687
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发表时间：
2011-02-01
期刊：
影响因子：
1.5
通讯作者：
Miquerol, Lucile
中科院分区：
文献类型：
--
作者：
Beyer, Sabrina;Kelly, Robert G.;Miquerol, Lucile
Connexin-40 （Cx40）基因编码一种间隙连接蛋白，在心房和心脏传导系统的心肌细胞以及大动脉内皮细胞的细胞间通讯中起重要作用。在胚胎发育过程中，Cx40的表达受到严格调控，并与进行性心室传导系统（VCS）分化和血管功能相关。我们通过靶向重组产生了携带CreERT2-IRESmRFP盒的Cx40（Cre）小鼠。在Cx40（Cre）小鼠中，RFP的表达模式与内源性Cx40基因和Cx40（GFP）等位基因的表达模式相同。使用基于lacz的Cre报告小鼠系，我们观察了他莫昔芬依赖性Cre重组在VCS和动脉内皮细胞中Cx40表达的时空分布。因此，Cx40（Cre）小鼠可用于对表达Cx40的细胞进行直接诱导性基因修饰。创世纪49:83 - 91,2011。(C) 2010 Wiley-Liss, Inc。
The Connexin-40 (Cx40) gene encodes a gap junction protein that plays an important role in cell-cell communication in cardiomyocytes of the atria and cardiac conduction system and endothelial cells of large arteries. During embryonic development, Cx40 expression is tightly regulated and correlates with progressive ventricular conduction system (VCS) differentiation and vessel function. We have generated Cx40(Cre) mice carrying a CreERT2-IRESmRFP cassette by targeted recombination. In Cx40(Cre) mice, the pattern of expression of RFP is identical to that of the endogenous Cx40 gene and a Cx40(GFP) allele. Using a LacZ-based Cre reporter mouse line, tamoxifen dependent Cre recombination was observed throughout the spatio-temporal profile of Cx40 expression in the VCS and arterial endothelial cells. Cx40(Cre) mice can therefore be used to direct inducible genetic modification in Cx40 expressing cells. genesis 49:83-91,2011. (C) 2010 Wiley-Liss, Inc.