Vascularized bone transplant chimerism mediated by vascular endothelial growth factor.
Vascularized bone transplant chimerism mediated by vascular endothelial growth factor.
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由血管内皮生长因子介导的血管化骨移植嵌合体。
DOI：
10.1002/micr.22300
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发表时间：
2015
期刊：
影响因子：
2.1
通讯作者：
Bishop,AllenT
中科院分区：
文献类型：
--
作者：
Willems,WouterF;Larsen,Mikko;Friedrich,PatriciaF;Bishop,AllenT
背景血管内皮生长因子（VEGF）在同种异体骨移植中诱导血管生成和成骨。我们的目的是确定是否在血管内皮生长因子治疗的骨同种异体移植的结果与循环衍生的自体细胞或生存的同种异体移植衍生cells.Methodsvascularized股骨骨移植移植从女性黑Agglutinarate大鼠（DA; RT 1a）移植到男性花斑病毒葛兰素（PVG; RT 1c）。动静脉束植入和短期免疫抑制用于维持细胞活力。实验组（n = 22）采用生物可降解微球包裹VEGF，并经髓内注射。在对照组（n = 22）中，不递送VEGF。在4周或18周处死大鼠。在内外皮质进行骨重建区域的激光捕获显微切割。用逆转录聚合酶链反应对性别不匹配的基因进行定量，以确定雄性细胞与总细胞的数量，定义为相对表达率（rER）. Results 4周时，VEGF治疗的移植物内皮层的rER显著高于未治疗的移植物（0.622 ± 0.225 vs. 0.362 ± 0.081，P = 0.043）。在4周时，对照组的外皮质具有显著更高的rER（P = 0.038），而VEGF组的内皮质具有更高的rER（P = 0.015）。随着时间的推移，VEGF治疗大鼠的外皮质rER在第18周显著增加至0.634 ± 0.106（P = 0.049）。18周时，两组所有皮质区域的rER均>0.5。结论这些体内研究结果表明，髓内应用的VEGF对受体来源的骨具有趋化作用，并可能意味着微囊化的VEGF可以使血管化同种异体移植物建立更快的血管生成。© 2014 Wiley Periodicals，Inc.显微外科35：45-51，2015年。
BackgroundVascular endothelial growth factor (VEGF) induces angiogenesis and osteogenesis in bone allotransplants. We aim to determine whether bone remodeling in VEGF‐treated bone allotransplants results from repopulation with circulation‐derived autogenous cells or survival of allogenic transplant‐derived cells.MethodsVascularized femoral bone transplants were transplanted from female Dark Agouti rats (DA;RT1a) to male Piebald Viral Glaxo (PVG;RT1c). Arteriovenous bundle implantation and short‐term immunosuppression were used to maintain cellular viability. VEGF was encapsulated in biodegradable microspheres and delivered intramedullary in the experimental group (n = 22). In the control group (n = 22), no VEGF was delivered. Rats were sacrificed at 4 or 18 weeks. Laser capture microdissection of bone remodeling areas was performed at the inner and outer cortex. Sex‐mismatched genes were quantified with reverse transcription‐polymerase chain reaction to determine the amount of male cells to total cells, defined as the relative expression ratio (rER).ResultsAt 4 weeks, rER was significantly higher at the inner cortex in VEGF‐treated transplants as compared to untreated transplants (0.622 ± 0.225 vs. 0.362 ± 0.081, P = 0.043). At 4 weeks, the outer cortex in the control group had a significantly higher rER (P = 0.038), whereas in the VEGF group, the inner cortex had a higher rER (P = 0.015). Over time, in the outer cortex the rER significantly increased to 0.634 ± 0.106 at 18 weeks in VEGF‐treated rats (P = 0.049). At 18 weeks, the rER was >0.5 at all cortical areas in both groups.ConclusionsThese in vivo findings suggest a chemotactic effect of intramedullary applied VEGF on recipient‐derived bone and could imply that more rapid angiogenesis of vascularized allotransplants can be established with microencapsulated VEGF. © 2014 Wiley Periodicals, Inc. Microsurgery 35:45–51, 2015.