Tomographic X-ray scattering based on invariant reconstruction: analysis of the 3D nanostructure of bovine bone.
Tomographic X-ray scattering based on invariant reconstruction: analysis of the 3D nanostructure of bovine bone.
复制标题
基于不变重构的x射线层析散射：牛骨三维纳米结构分析。
DOI：
10.1107/s1600576721000881
复制
发表时间：
2021-04-01
影响因子：
6.1
通讯作者：
Fratzl P
中科院分区：
文献类型：
--
作者：
De Falco P;Weinkamer R;Wagermaier W;Li C;Snow T;Terrill NJ;Gupta HS;Goyal P;Stoll M;Benner P;Fratzl P
提出了一种新的层析方法，使用X射线散射，允许表征的混合材料的三维纳米结构。小角X射线散射（SAXS）是一种有效的表征多相纳米复合材料的技术。生物材料的结构复杂性和异质性要求开发新的技术来对其分层结构进行3D表征。新兴的SAXS层析成像方法允许在每个体素中重建3D散射图案，但在同步加速器测量时间和计算机时间方面是昂贵的。为了解决这个问题，已经开发了一种方法的基础上重建的SAXS不变量，以允许快速的三维表征的纳米结构的非均匀材料。SAXS不变量是替代每个体素中的3D散射图案的标量，从而将6D重建问题简化为几个3D重建问题。断层重建的标准程序可以直接适用于这个问题。该程序是通过确定整个宏观的3D体积的牛皮质骨的纳米骨矿物质颗粒厚度（T参数）的分布。T参数图显示纤维板层骨单位中颗粒厚度的空间模式。矿物纳米颗粒结构和微观特征之间的空间相关性表明，矿物颗粒在血管通道附近特别薄。
A new tomographic approach using X-ray scattering is presented, allowing the characterization of the 3D nanostructure of hybrid materials. Small-angle X-ray scattering (SAXS) is an effective characterization technique for multi-phase nanocomposites. The structural complexity and heterogeneity of biological materials require the development of new techniques for the 3D characterization of their hierarchical structures. Emerging SAXS tomographic methods allow reconstruction of the 3D scattering pattern in each voxel but are costly in terms of synchrotron measurement time and computer time. To address this problem, an approach has been developed based on the reconstruction of SAXS invariants to allow for fast 3D characterization of nanostructured inhomogeneous materials. SAXS invariants are scalars replacing the 3D scattering patterns in each voxel, thus simplifying the 6D reconstruction problem to several 3D ones. Standard procedures for tomographic reconstruction can be directly adapted for this problem. The procedure is demonstrated by determining the distribution of the nanometric bone mineral particle thickness (T parameter) throughout a macroscopic 3D volume of bovine cortical bone. The T parameter maps display spatial patterns of particle thickness in fibrolamellar bone units. Spatial correlation between the mineral nanostructure and microscopic features reveals that the mineral particles are particularly thin in the vicinity of vascular channels.