FecB mutation and litter size are associated with a 90-base pair deletion in BMPR1B in East Friesian and Hu crossbred sheep
FecB mutation and litter size are associated with a 90-base pair deletion in BMPR1B in East Friesian and Hu crossbred sheep
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DOI：
10.1080/10495398.2021.2020805
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发表时间：
2022-01-04
影响因子：
3.7
通讯作者：
Song, Yuxuan
中科院分区：
文献类型：
--
作者：
Li, Danni;Zhang, Lei;Song, Yuxuan
产仔数是牲畜的一个重要经济性状，但只有少数研究关注 BMPR1B 的相关插入缺失突变，BMPR1B 是绵羊排卵和产仔数的关键调节因子。我们评估了 BMPR1B 突变对绵羊繁殖性能的影响。我们使用胡羊、东弗里斯兰羊和东弗里斯兰/胡杂交羊作为实验对象，并在 BMPR1B 中发现了一个新的 90 bp 缺失，这与 c.746A > G（FecB 突变）基因型一致。然后评估两个基因座和窝产仔数之间的相关性。我们鉴定了 Del-90bp 基因座的三种基因型，即 II、ID 和 DD，以及 c.746A > G 基因座的三种基因型，即 ++、B+ 和 BB。 Del-90bp和c.746A>G均显着影响Hu和东弗里斯兰/Hu杂交羊的产羔数。连锁不平衡分析显示湖羊和F1群体中这些位点之间存在很强的连锁不平衡(r(2) > 0.33)，这表明检测该90 bp缺失可能是识别c.746A > G可能携带者的简单方法。然而，该90 bp缺失的功能仍需要进一步探索。我们提供的遗传数据可作为改良绵羊多产性状育种的参考。
Litter size is a critical economic trait in livestock, but only a few studies have focused on associated indel mutations in BMPR1B, a key regulator of ovulation and litter size in sheep. We evaluated the effects of BMPR1B mutations on the reproductive performance of sheep. We used Hu, East Friesian, and East Friesian/Hu crossbred sheep as experimental subjects and identified a novel 90 bp deletion in BMPR1B, which coincides with the c.746A > G (FecB mutation) genotype. The correlation between the two loci and litter size was then evaluated. We identified three genotypes for the Del-90bp locus, namely, II, ID, and DD, and three genotypes for the c.746A > G locus, namely ++, B+, and BB. Both Del-90bp and c.746A > G significantly affected the litter size of Hu and East Friesian/Hu crossbred sheep. Linkage disequilibrium analysis revealed a strong linkage disequilibrium between these loci in Hu sheep and the F1 population (r(2) > 0.33), which suggests that detecting this 90 bp deletion might be a simple method to identify the likely carriers of c.746A > G. However, the function of this 90-bp deletion still needs further exploration. We provide genetic data that can be used as a reference for the breeding of improved prolific traits in sheep.