MoARG1, MoARG5,6 and MoARG7 involved in arginine biosynthesis are essential for growth, conidiogenesis, sexual reproduction, and pathogenicity in Magnaporthe oryzae
MoARG1, MoARG5,6 and MoARG7 involved in arginine biosynthesis are essential for growth, conidiogenesis, sexual reproduction, and pathogenicity in Magnaporthe oryzae
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参与精氨酸生物合成的 MoARG1、MoARG5,6 和 MoARG7 对于稻瘟病菌的生长、分生孢子发生、有性生殖和致病性至关重要
DOI：
10.1016/j.micres.2015.07.002
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发表时间：
2015-01-01
影响因子：
6.7
通讯作者：
Lin, Fucheng
中科院分区：
文献类型：
--
作者：
Zhang, Yong;Shi, Huanbin;Lin, Fucheng
精氨酸是真核生物细胞中功能最丰富的氨基酸之一，在蛋白质合成、氮代谢、一氧化氮（NO）和尿素生物合成等过程中发挥重要作用。精氨酸的从头合成途径在真菌界中是保守的，但在植物病原真菌中的了解甚少。本研究对稻瘟病菌精氨酸生物合成第七步、第二-第三步和第五步的3个合成酶编码基因MoARG1、MoARG5、6和MoARG7的功能进行了研究。缺失MoARG1或MoARG5，6，导致精氨酸营养缺陷型突变体，其对基本培养基（MM）上的精氨酸有严格的要求。DeltaMoarg1和DeltaMoarg5、6的气生菌丝生长、色素沉着、分生孢子发生、有性生殖和致病性均严重降低。有趣的是，像酿酒酵母一样，MoARG7的缺失导致精氨酸营养缺陷型渗漏，并减弱致病性。有限的附着胞介导的渗透和限制入侵菌丝生长在宿主细胞是负责严重减弱的致病性的Arg（-）突变体。此外，我们还对Arg（-）突变体和野生型的孢子萌发和附着胞形成过程中NO的产生进行了监测，证明了NO的产生可能不是通过依赖于精氨酸的途径。米。总之，MoARG1、MoARG5、6和MoARG7是M.米。(C)2015 Elsevier GmbH. All rights reserved.
Arginine is one of the most versatile amino acids in eukaryote cells, which plays important roles in a multitude of processes such as protein synthesis, nitrogen metabolism, nitric oxide (NO) and urea biosynthesis. The de novo arginine biosynthesis pathway is conserved among fungal kingdom, but poorly understood in plant pathogenic fungi. Here, we characterized the functions of three synthetic enzyme-encoding genes MoARG1, MoARG5,6, and MoARG7, which involved the seventh step, second-third step and fifth step of arginine biosynthesis in Magnaporthe oryzae, respectively. Deletion of MoARG1 or MoARG5,6, resulted in arginine auxotrophic mutants, which had a strict requirement for arginine on minimal medium (MM). Both Delta Moargl and Delta Moarg5,6 severely reduced in aerial hyphal growth, pigmentation, conidiogenesis, sexual reproduction and pathogenicity. Interestingly, like Saccharomyces cerevisiae, deletion of MoARG7 caused a leaky arginine auxotrophy, and attenuated pathogenicity. Limited appressorium-mediated penetration and restricted invasive hyphae growth in host cells are responsible for the severely attenuated pathogenicity of the Arg(-) mutants. Additionally, we monitored the NO generation during conidial germination and appressorial formation in both Arg(-) mutants and wild type, and demonstrated that NO generation may not occur via arginine-dependent pathway in M. oryzae. In summary, MoARG1,MoARG5,6, and MoARG7 are required for growth, conidiogenesis, sexual reproduction, and pathogenicity in M. oryzae. (C) 2015 Elsevier GmbH. All rights reserved.