Drinking spout orifice size affects licking behavior in inbred mice
Drinking spout orifice size affects licking behavior in inbred mice
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DOI：
10.1016/j.physbeh.2005.06.010
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发表时间：
2005-08-07
影响因子：
2.9
通讯作者：
Spector, AC
中科院分区：
文献类型：
--
作者：
Dotson, CD;Spector, AC
使用舔液计，我们评估了饮水口尺寸对近交小鼠舔行为的影响 [C57BL/6J、SWR/J、129P3/J 和 DBA/2J]。动物从具有被定义为大（2.7毫米）或小（1.5毫米）孔口的饮水嘴吸管中舔食。在单独的 30 分钟会话中，小鼠从固定的单个小孔饮水嘴中舔的次数大约是从带有大孔的饮水嘴中舔的次数的两倍。然而，它们的总摄入量大致相同。我们计算出，小鼠从小孔饮水管平均每舔 0.55 μL，从大孔饮水管平均每舔 1.15 μL。因此，动物似乎通过根据舔体积的函数按比例调整其舔舐来调节其液体摄入量。平均而言，这种调节是通过调节舔舐爆发的大小而不是其频率来实现的。然而，观察到补偿策略的应变差异。当舔舐仅限于 30 分钟简短访问测试中的一系列 5 秒试验时，较小的孔口尺寸增加了所表达的响应范围。小鼠每次尝试的平均舔次数增加了 20%，而当从带有小孔的喷嘴进行舔时，小鼠的平均舔次数增加了 60%。有趣的是，当孔口尺寸在短暂的访问过程中准随机变化时，大孔口饮水嘴的舔舐作用更大，这表明从两种孔口尺寸输送的水在其增强功效方面不同。这些发现表明，饮水嘴孔的大小可以显着影响小鼠舔舐测试的实验结果，并且在测试味觉或其他因素对摄入行为的影响时应注意控制该变量。 (c) 2005 Elsevier Inc. 保留所有权利。
Using a lickometer, we assessed the effect of drinking spout orifice size on the licking behavior of inbred mice [C57BL/6J, SWR/J, 129P3/J and DBA/2J]. Animals licked from drinking spout sipper tubes that had what were defined as either a large (2.7 mm) or a small (1.5 mm) orifice. Mice took approximately twice as many licks from a stationary single small orifice drinking spout than when licking from a spout with a large orifice during separate 30-min sessions. However, their total intake volume was approximately the same. We calculated that mice received a mean of 0.55 mu L per lick from the drinking tubes with a small orifice and a mean of 1.15 mu L per lick from the drinking tubes with a large orifice. Thus, the animals appear to have regulated their fluid intake by proportionally adjusting their licking as a function of the lick volume. On average, this regulation occurred through modulation of the size of licking bursts and not their frequency. However, strain differences in compensation strategy were observed. When licking was restricted to a series of 5-s trials in a 30-min brief access test session, the smaller orifice size increased the range of responsiveness that was expressed. Mice increased their average licks per trial by 20% and took 60% more trials when licking from a spout with a small orifice. Interestingly, when the orifice size was quasi-randomly varied within a brief access session, licking was greater from large orifice drinking spouts, suggesting that water delivered from the two orifice sizes differs in its reinforcement efficacy. These findings demonstrate that drinking spout orifice size can significantly influence experimental outcomes in licking tests involving mice and care should be taken in controlling this variable in testing the effects of taste or other factors on ingestive behavior. (c) 2005 Elsevier Inc. All rights reserved.