Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.
Malignancies of metastatic murine lymphosarcoma cell lines and clones correlate with decreased cell surface display of RNA tumor virus envelope glycoprotein gp70.
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转移性鼠淋巴肉瘤细胞系和克隆的恶性肿瘤与 RNA 肿瘤病毒包膜糖蛋白 gp70 的细胞表面展示减少相关。
DOI：
10.1073/pnas.77.10.5943
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发表时间：
1980
影响因子：
11.1
通讯作者：
G. Nicolson
中科院分区：
文献类型：
--
作者：
C. Reading;K. Brunson;M. Torrianni;G. Nicolson
鼠淋巴肉瘤RAW 117的变异亚系已经通过在同基因BALB/c小鼠中静脉内接种细胞和收获实体肝肿瘤的连续循环而得到[Brunson K. W. & Nicolson，G. L.（1978）J. Natl. Cancer Inst.61，1499-1503]以及通过在固定化凝集素上重复吸附依次去除凝集素反应性细胞[阅读，C. L. Belloni，P. N. & Nicolson，G. L.等人（1980）J. Natl. Cancer Inst.64，1241-1249]。这些细胞亚系和它们的克隆进行了分析的能力，以形成总肝肿瘤转移后，静脉注射或皮下注射到同系小鼠，这种反应是有关的某些细胞表面特性，包括病毒抗原和凝集素结合位点的数量，暴露的特定细胞表面蛋白，和细胞表面糖蛋白的数量可视化凝胶与125碘标记的凝集素或抗体。根据凝胶系统，在低恶性和高恶性的RAW 117亚系之间，细胞表面糖蛋白组分的量或暴露量约为70，000或69，000和71，000。RAW 117系和克隆中RNA肿瘤病毒抗原的竞争放射免疫测定表明存在莫洛尼鼠白血病病毒抗原gp 70、p30和p12。增强的恶性肿瘤和转移到肝脏伴随着病毒抗原的细胞内容物的减少和gp 70细胞表面暴露的损失。从体内和体外选择的高或低恶性度的亚系获得的几个克隆的分析证实了在该系统中转移和gp 70表达之间的反比关系。
Variant sublines of the murine lymphosarcoma RAW117 have been derived by sequential cycles of intravenous inoculation of cells and harvesting of solid liver tumors in syngeneic BALB/c mice [Brunson K. W. & Nicolson, G. L. (1978) J. Natl. Cancer Inst. 61, 1499-1503] and also by sequential removal of lectin-reactive cells via repeated adsorption on immobilized-lectins [Reading, C. L. Belloni, P. N. & Nicolson, G. L. (1980) J. Natl. Cancer Inst. 64, 1241-1249]. These cell sublines and their clones were analyzed for abilities to form gross liver tumor metastases after injection intravenously or subcutaneously into syngeneic mice, and this response was related to certain cell surface properties including quantities of viral antigens and lectin-binding sites, exposure of specific cell surface proteins, and quantities of cell surface glycoproteins visualized in gels with 125I-labeled lectins or antibodies. Consistent differences were obtained between RAW117 sublines of low and high malignancy with respect to the amounts or exposures of cell surface glycoprotein components of Mr approximately 70,000 or 69,000 and 71,000, depending on the gel system. Competition radioimmunoassays for RNA tumor virus antigens in the RAW117 lines and clones indicated the presence of Moloney murine leukemic virus antigens gp70, p30, and p12. Enhanced malignancy and metastasis to liver was accompanied by decreases in the cellular contents of viral antigens and loss of gp70 cell surface exposure. Analysis of several clones obtained from sublines selected in vivo and in vitro for high or low malignancy confirmed the inverse relationship between metastasis and expression of gp70 in this system.